Applying biofluid 1H NMR-base metabonomic 课件.ppt

Applying biofluid 1H NMR-base metabonomic techniques to distinguish between HIV-1 positive/AIDS patients on antiretroviral treatment and HIV-1 negative individuals应用1H NMR基于代谢组学技术分析人体内的液体来区分HIV-1阳性或用抗反转录病毒治疗的爱滋病人和HIV-1阴性的病人 文章来源:Journal of Pharmaceutical and Biomedical Analysis 41 (2006) 1442–1446 作者: R. Hewer, J. Vorster, F.E. Steffens, D. Meyera,? Abstract  (摘 要) ※ Metabonomics, the study of metabolites and their role in various physiological states, is a novel methodology arising from the post-genomics era and has extensive biomedical application. This technology has exhibited vast success in the identification and study of human diseases and may find further application in the study of HIV/AIDS. Specifically, the wide range of clinical and metabolic abnormalities associated with the use of antiretroviral (ARV) treatment may be investigated. ※ To this end, this preliminary study evaluated whether metabonomic techniques could distinguish between HIV-1 positive/AIDS patients utilizing antiretroviral therapy and HIV-1 negative individuals. Serum metabolic profiles determined by 1H nuclear magnetic resonance (NMR) spectroscopy combined with pattern recognition analysis of the data showed that this distinction was attainable; suggesting that ARV-associated side-effects could be monitored utilizing NMR metabonomic techniques. Introduction  (引 言) 1、1H NMR spectroscopy of biofluids (urine, serum/plasma) and tissue generates comprehensive biochemical profiles of low molecular weight endogenous metabolites。 2、Metabonomics have been applied to both diseased and healthy。 3、Notable metabonomic applications include the distinction between type 2 diabetic patients and healthy volunteers， diagnosis of coronary heart disease and investigation of hypertension。 4、1H NMR spectroscopy-based metabonomics could find extensive application in the monitoring of the biological side-ef