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GC法同时测定芎菊上清片中4成分的含量 (1.抚顺市食品药品检验所，抚顺113000；2.辽宁省食品药品检验所，沈阳110023) 摘要 目的：建立同时分析测定芎菊上清片中4个活性成分(α-松油醇、薄荷酮、薄荷脑和胡薄荷酮)的方法。方法：采用GC法，色谱柱为DB-1701毛细管色谱柱(30 m×0.3 mm×0.25 μm)，柱温℃保持min，℃·min-1的速率升至120℃，保持min，再以0 ℃·min-1的速率升温至220 ℃，保持2,进样口温度℃,FID检测器检测器温度℃。结果：α-松油醇、薄荷酮、薄荷脑和胡薄荷酮线性范围分别为0.01～0.55mg·mL-1(r=0.9999)0.10～0. 5mg·mL-1 (r=0.9998)、 0.01~0. 50mg·mL-1 (r=0.9998)、 0.01~0.51mg·mL-1(r=0.9999);平均加样回收率(n=6)分别为%、98.2%、95.7%、96.1%，其RSD分别为1.%、1.4%、1.6%、1.6%。结论：该方法操作简单，重复性好，可有效控制芎菊上清片的质量。 关键词：芎菊上清片；气相色谱法；含量测定GC Simultaneous Determination of Four Volatile Components in Xiongjushangqing Tablets Luo Jing1 ， Shi Fa2 ， Kong Lingfeng2 ， Ni Long3 (1. Fushun Institute for Food and Drug Control, Fushun 113000;2. Liaoning Institute for Food and Drug Control, Shenyang 110023；3. Chinese Pharmacopoeia Commission, Beijing 100061) Abstract Objective: To establish a method for simultaneous determination of four active ingredients(α- terpineol, menthone, menthol, pulegone) in Xiongjushangqing Tablets. Methods: GC method was adopted .The determination was performed on a DB-1701 column(30 m×0.25 mm×0.25 μm). The column temperature was programmed (maintained at 80 ℃ for 1 min, then risen to 120 ℃ at the rate of 5℃·min-1, held for 5 min. After that the temperature was elevated to 220 ℃ at the rate of 50 ℃·min-1and held for 2 min).The FID was used as detector, the temperatures of the injection and the detector were set at 200 ℃ and 230 ℃. Results:The calibration curves were linear in the ranges of 0.01–0.55 mg·mL-1 for α- terpineol(r=0.9999), 0.10–0.50mg·mL-1 for menthone(r=0.9998), 0.01–0.50 mg·mL-1 for menthol (r=0.9998) and 0.01–0.51mg·mL-1 for pulegone(r=0.9999). The average recoveries(n=6) were 97.6%，98.2%，95.7% and 96.1% RSD were 1.5%，1.4%，1.6% and 1.6%, respectively. Conclusion:The method is simple and repeatable,which can be used for quality control of Xiongjushangqing ablets. Key words: Xiongjushangqing Tablets； GC； volatile component； assay 芎菊上清收载于中国药典2010年版一部由等味中药材清热解表，散风止痛用于外感风邪引 [1] 。中〔含