医学论文-排脓散活性成分对小鼠的抗炎作用及其配伍的定量研究.docVIP

医学论文-排脓散活性成分对小鼠的抗炎作用及其配伍的定量研究 ?????????????????????? 作者：陈君超, 李禄金, 文世梅, 何迎春, 刘红霞, 郑青山 【摘要】? 目的：观察排脓散各药味部分活性成分的抗炎作用，并对其不同配伍进行定量研究。方法：选择排脓散中的部分活性组分芍药苷、柚皮苷加新陈皮苷、桔梗皂苷，用正交设计法安排实验，剂量两水平分别为用药和不用药。选择醋酸所致小鼠腹腔毛细血管通透性亢进为急性炎症模型，以阿司匹林和生理盐水为对照，以对毛细血管通透性影响作为炎症指标，定量分析各药味活性成分的抗炎作用及相互作用。结果：全方活性成分组合（柚皮苷加新陈皮苷、芍药苷、桔梗皂苷）对醋酸所致炎症渗出的抑制效果与阿司匹林组比较，差异无统计学意义（P0.05），药效模拟结果提示为最大效应组合。与生理盐水组比较，排脓散中各药味活性成分及其他不同配伍组合均不同程度抑制炎症的渗出（P0.01）。复方配伍的预测效应区间为0.115～0.170（光密度值），全部活性成分组合呈现协同作用；各活性成分对复方的贡献按重要程度排列为：芍药苷柚皮苷加新陈皮苷桔梗皂苷。 结论：排脓散各药味活性成分对抑制小鼠腹腔毛细血管通透性均呈正性作用，芍药苷贡献度最大，全方活性成分组合抑制作用最强。采用正交模拟法对复方配伍进行定量分析，有可能挖掘出更多的复方配伍信息。 【关键词】? 排脓散; 活性成分; 中药配伍; 药物相互作用. ??? Methods: The mouse model of acute inflammation with increase of capillary permeability was induced by intraperitoneal injection of acetic acid. An orthogonal design with 2 levels (used and unused) was applied to assign the combinations groups of active ingredients including naringin and neohesperidin, peoniflorin, and platycodin. Aspirin and normal saline were administered as control. The pharmacodynamic interactions were analyzed by the optical density (OD) of infiltrated Evans blue. ??? Results: The different combinations of active ingredients showed antiinflammatory effect with different degree, and the predicted values of OD varied from 0.115 to 0.170. The maximum antiinflammatory effect was from the combination of naringin, neohesperidin, paeoniflorin and platycodin, better than that of the saline group (P0.01). However, there was no significant difference as compared with the aspirin group (P0.05). Paeoniflorin showed a dominant contribution to the formula, and platycodin the least. The combination of all active components exhibited synergism. ??? Conclusion: The results suggest that all the ingredients are efficacious constituents of the formula, and paeoniflorin shows a dominant contribution to the formula. More information about prescription compatibility can be obtained by the orthogonal simulation method. ??? Keywords: Painong powder; active ingredi