医学论文-去细胞牛松质骨复合骨髓基质干细胞修复兔桡骨缺损.docVIP

医学论文-去细胞牛松质骨复合骨髓基质干细胞修复兔桡骨缺损 ?????????????????作者：王德超 郑玉琴 孙建华 史晨辉 董金波 刘维钢? 【摘要】? 目的：研制理想的能够修复骨缺损的骨材料，为其在骨缺损修复领域中的临床应用提供实验依据。方法：体 外扩增诱导兔骨髓基质干细胞，将其与去细胞牛松质骨复合培养。造成兔桡骨中段 10 mm 骨缺损模型，分复合细胞组、单纯材料组、空白对照组。通过大体观察、放射学检查、组织学检查观察骨缺损的修复情况。结果：去细胞牛松质骨复合骨髓基质干细胞修复兔桡骨缺损，12 周时缺损区完全被新骨代替，骨髓腔完全通畅，优于单纯材料组的修复效果，单纯材料组优于空白对照组。结论：去细胞牛松质骨具有良好的成骨能力，有可能成为理想的骨支架材料。 【关键词】? 骨修复材料；去细胞牛松质骨；组织工程 ??? 〔Abstract〕??? Objective???? To? prepare? an? ideal? scaffold? material? for? the? repair? of? bone? defect,? for? the? clinical? use? in? the? bone? reconstruction? field.??? Methods???? Rabbit? marrow? stem? cells? (MSCs)? were? cultured? and? expanded? in? vitro.? The? MSCs? were? mixed? with? acellular? bovine? cancellous? bone? (ABCB).? The? rabbits? were? prepared? as? model? of? bone? defects,? divided? into? 3? groups:named? complex? group,? acellular? bovine? cancellous? bone? group,? and? blank? control.? The? animals? were? sacrificed? and? new? bone? regeneration? in? the? defects? was? investigated? by? gross? specimen? inspection,? X-ray? examination? and? histological? observation.??? Results???? At? 12? weeks,? the? radial? defects? repaired? by? named? complex? group,? and? their? marrow? cavities? formed,? which? is? better? than? acellular? bovine? cancellous? bone? group.? Acellular? bovine? cancellous? bone? group? is? better? than? blank? control.??? Conclusion???? Acellular? bovine? cancellous? bone? may? be? used? as? a? kind? of? ideal? scaffold? material. ??? 〔Key? Words〕??? Bone? repair? material;? Acellular? bovine? cancellous? bone;? Bone? tissue? engineering ??? 　　研制理想的生物材料替代自体骨移植用于修复骨组织缺损是医学和生物材料科学领域的一个重要课题。目前临床上应用的骨移植物较多，如羟基磷灰石、脱钙骨、脱蛋白骨等为临床骨缺损的治疗提供了很多选择。但是这些方法各有优缺点，难以满足临床上修复各种骨缺损的需要。去细胞牛松质骨作为新型的生物衍生支架材料，具有良好的力学性质、空间结构、降解性、生物相容性等特点，日益受到重视。本研究将兔骨髓间充质干细胞（MSCs）与去细胞牛松质骨载体相结合，构建组织工程骨，探索修复兔桡骨节段性骨缺损的可行性。 ??? 1??? 材料与方法 ??? 1.1??? 材料 ??? 1.1.1??? 材料与试剂??? 新鲜牛肱骨（石河子市农贸市场），脱细胞的各种试剂（曲拉通 X-100 由上海生物工程公司提供，氯化钠由西安化学试剂厂提供）， HWY 100B 型恒温培养摇床（上海智城分析仪器制造有限公司），Massion 染色试剂盒（广州市俪科贸易有限公司），新西兰大白兔（