医学论文-1,25-二羟维生素D3对链脲菌素诱导的小鼠1型糖尿病预防作用及机制探讨.docVIP

医学论文-1,25-二羟维生素D3对链脲菌素诱导的小鼠1型糖尿病预防作用及机制探讨 【摘要】? 　　 目的 1,25-二羟维生素D3［1,25-(OH)2D3］对连续多次小剂量链脲菌素（the multiple low dose streptozotocin,MLDS）诱导的1型糖尿病小鼠的预防作用及其机制初探。方法 MLDS腹腔注射制备1型糖尿病小鼠模型，以血糖水平持续高于16.7mmol/L为成模标准。实验共分三组，分别为正常对照组、糖尿病组、预防组。采用便携式血糖仪监测各组血糖水平；放射免疫法检测各组血清胰岛素含量；免疫组化法检测各组胰岛细胞凋亡相关蛋白Bax和Bcl-xL表达。结果 MLDS诱发的1型糖尿病模型在第四周基本建成。MLDS使小鼠血糖升高，血清胰岛素含量下降，Bax上调、Bcl-xL下调。预防组血糖水平下降，血清胰岛素升高，Bax下调、Bcl-xL上调，糖尿病发病率下降，与糖尿病组比较有显著差异(P＜0.05)。结论 1,25-(OH)2D3可有效预防MLDS诱导的1型糖尿病的发生，其效应可能与1,25-(OH)2D3调节Bax和Bcl-xL蛋白表达平衡有关。 【关键词】? 1,25-二羟维生素D3；链脲菌素；1型糖尿病；Bax；Bcl-xL ??? Abstract：Objective To investigate the prevention and the mechanism of 1,25-dihydroxyvitamin D3 to type 1 diabetes by streptozotocin-induced in KM mice.Methods Experimental type 1 diabetes mice model with the blood glucose level which was consistently higher than 16.7mmol/L were produced by intraperitoneal injection of MLDS.There are three groups in this studay,which were normal control group,diabetes group,and 1,25-dihydroxyvitamin D3 prevention group.Blood glucose level was monitored using a one-touch blood glucose meter.Radioimmunnity analysis was used to detect the serum insulin level.The protein expression level of bax and bcl-xL in islets were detected with immunohistochemical technique.Results MLDS-induced type 1 diabetes mice model was finished in the fourth week.Blood glucose level and bax protein expression level increased after the treatment of MLDS,but serum insulin level and bcl-xL protein expression level decreased.1,25-dihydroxyvitamin D3 prevented diabetes induced by MLDS in KM mice.The blood glucose level decreased,the serum insulin level increased.Bcl-xL protein expression was up regulated,bax protein expression was down regulated and the incidence of diabetes decreased significantly in 1,25-dihydroxyvitamin D3 prevention group compared with the control.Conclusion 1,25-dihydroxyvitamin D3 treatment could prevent type 1 diabetes through regulating the protein expression of bax and bcl-xL. ??? Key words:1,25-dihydrox