医学论文-STZ 建立 2 型糖尿病大鼠模型的剂量探讨.docVIP

医学论文-STZ 建立 2 型糖尿病大鼠模型的剂量探讨 【摘要】? 目的：探讨链脲佐菌素（streptozocin, STZ）腹腔注射建立 2 型糖尿病（T2DM）大鼠模型的最佳注射剂量。方法：4 周龄 SPF 级雄性 SD 大鼠高糖高脂饲料喂养 4 周，分别按 30 mg/kg、40 mg/kg、50 mg/kg 体重剂量腹腔注射 STZ 建立 T2DM 大鼠模型，观察成模大鼠各阶段体重、食量、饮水量、尿量变化；检测空腹血糖（FBG）、胰岛素（FINS）、血脂，比较各组大鼠模型稳定性。结果：40 mg/kg 剂量组大鼠的成模率最高（73.3%），成模大鼠具有胰岛素抵抗、高血糖、高胰岛素血症和血脂紊乱的 T2DM 临床特征，高血糖持续时间最长、最稳定（到实验结束模型稳定至少 28 d）。30 mg/kg 剂量组大鼠成模率只有 40%，成模大鼠于实验结束血糖水平已基本恢复正常，50 mg/kg 剂量组成模大鼠倾向于 1 型糖尿病。结论：短期高糖高脂饮食联合 STZ 腹腔注射建立 2 型糖尿病 SD 大鼠模型的 STZ 最佳注射剂量为 40 mg/kg。 【关键词】? 2 型糖尿病；大鼠模型；链脲佐菌素 ??? 〔Abstract〕 Objective To? explore? the? best? dosage? of? streptozocin? (STZ)? what? was? used? to? induce? rat?? model? of?? Type? 2? diabetes? mellitus? (T2DM).? Methods?? Sixty? 4? weeks? old? male? Sprague? Dawley? (SD)? rats? were? fed? with? high? fatty? and? high? sugar? diet? for? 4? weeks,? then? STZ? (30? mg/kg、40? mg/kg、50? mg/kg)? was? injected? intraperitoneally? to? make? rat? model? of? T2DM,? each? phase? avoirdupois,? appetite? and? the? amount? of? urine? and? drinking? of? rat? model? were? tested,? in? unison,? the? fasting? blood? glucose? level? (FBG),? serum? insulin? level? (FINS)? and? blood? lipid? were? also? detected,? the? data? was? used? to? evaluate? the? stability? of? the? rat? model? of? T2DM.??? Results? The? 40? mg/kg? group? has? the? highest? making? model? success? rate? of? over? 73.3%,? all? the? succeed? model? rat? has? insulin? resistance,? hyperglycemia,? blood? lipid? disorder? and? blood? insulin? increased? which? were? the? typical? characteristic? of? T2DM,? and? duration? of? high? blood? glucose? was? the? longest? and? the? most? stabile? too.? Only? 40%? of? the? 30? mg/kg? group? has? succeed,? beside? the? blood? glucose? level? has? get? right? until? the? end? of? experimental.? The? successful? rat? models? of? the? 50? mg/kg? group? were? inclined? to? type? 1? diabetes? mellitus.?? Conclusion? Associate? with? high? fatty? and? high? sugar? diet? feeding? shortly,? the? best? dosage? of? STZ? which? was