医学论文-多种环境因素改变对心肌缺血家兔血浆内皮素的影响.docVIP

医学论文-多种环境因素改变对心肌缺血家兔血浆内皮素的影响 【摘要】? 目的 观察多因素环境变化对心肌缺血家兔血浆内皮素的影响。方法 将90只健康家兔随机分为正常对照组、单纯饥饿组、单纯低氧组、单纯高温组、单纯心肌缺血组、心肌缺血低氧组、心肌缺血饥饿组、心肌缺血低氧高温组和心肌缺血低氧高温饥饿组，每组10只。心肌缺血按心肌缺血方法进行。把各组分别放入相应的环境中，时间均为30min。实验结束后取动脉血，测血浆内皮素的含量。结果 除单纯饥饿组和单纯高温组外，其余各组内皮素均明显高于正常对照组（P＜0.05或0.01）。多种环境因素变化均使心肌缺血家兔内皮素有不同程度的升高，特别是心肌缺血低氧高温饥饿组的升高最为明显（P＜0.01）。结论 多环境因素变化对心肌缺血家兔血浆内皮素有明显的影响。 【关键词】? 环境因素诱发疾病；心肌缺血；内皮缩血管肽类；兔；动物实验 ??? Abstract:? Objective? To observe the changes of endothelin (ET) in rabbits with myocardial ischemia under different circumstances.? Methods? 90 healthy rabbits were placed in different anoxia-containers or hyperthermia-tanks for 30 minutes. Based on these, they were randomly allocated to 9 groups, 10 in each group: control group, hunger group, anoxia group, hyperthermia group, myocardial ischemia group, myocardial ischemia plus anoxia group, myocardial ischemia plus hunger group,? myocardial ischemia plus anoxia plus hyperthermia group, myocardial ischemia? plus anoxia plus hyperthermia plus hunger group. The myocardial ischemia models were obtained according to traditional methods. 30 min after the rabbits were placed in corresponding containers, the artery blood was obtained to determine plasma ET.? Results? Except hunger group and hyperthemia group, the endothelin levels in other groups were higher? than that in control group, there were statistically differences (P0.05 or 0.01). Different circumstances enhanced the elevation of plasma ET in rabbits with myocardial ischemia to different extent, especially found in myocardial ischemia plus anoxia plus hyperthermia plus hunger group, compared to myocardial ischemia group, there was statistically significant difference (P0.01).? Conclusion? Various surrounding factors affect plasma ET in rabbits with myocardial ischemia.????????? 　　Key words:? disorders of environmental origin; myocardial ischemia; endothelins; rabbits; animal experiments 　　内皮素（ET）是心肌损伤的一重要因素，其升高幅度与心肌损伤严重程度相关，其高低可反映心肌缺血严重程度的指标［1］。我们曾研究发现心肌缺血后在低氧和低温情况下ET升高［2］。但是高温、饥饿和