医学论文-不同治法方药对脂肪肝大鼠肝组织NFκBp65及Kupffer细胞p38MAPK蛋白表达的影响.docVIP

医学论文-不同治法方药对脂肪肝大鼠肝组织NFκBp65及Kupffer细胞p38MAPK蛋白表达的影响 ????????????????作者：杨钦河，谢芳，王凤珍，凌家生，王强，程少冰，唐海兰， ?????????????胡巢凤，王彦平，孙升云，金玲，周羽竝，陈万群，杨环文，张玉佩 【摘要】? 　　【目的】探讨中医不同治法方药对脂肪肝大鼠肝组织核因子κB(NFκBp65)及Kupffer细胞p38MAPK蛋白活性的影响。【方法】选用SD大鼠98只，随机分为正常组、模型组、疏肝组(灌服32 g·kg-1·d-1剂量的柴胡疏肝散)、健脾组(灌服100 g·kg-1·d-1剂量的参苓白术散)、祛湿组(灌服35 g·kg-1·d-1剂量的平胃散)、活血组(灌服71 g·kg-1·d-1剂量的膈下逐瘀汤)、综合组(灌服119 g·kg-1·d-1剂量的自拟方);采用高脂饲料喂养、白酒灌胃法复制大鼠脂肪肝实验动物模型，给药12周后处死动物取肝组织，应用免疫组织化学法检测NFκBp65在各组中的活性及组织病理学变化。同时每组取3只大鼠分离Kupffer细胞，应用Western blot法检测不同组别大鼠Kupffer细胞p38MAPK蛋白的表达及其磷酸化水平的变化。【结果】模型组大鼠肝组织NFκBp65蛋白表达较正常组显著增加(P＜005)，疏肝组、健脾组NFκBp65阳性表达最低、肝组织脂肪变最轻，与模型组比较差异有显著性意义(P005)，且脂肪肝大鼠肝组织NFκBp65表达强度与其病理学的改变呈正相关(P001)。模型组大鼠Kupffer细胞p38MAPK蛋白表达和磷酸化p38MAPK蛋白表达较正常组显著增加(P001)，各给药组p38MAPK蛋白表达和磷酸化p38MAPK蛋白表达均较模型组显著降低(P001)，其中以健脾组、疏肝组下降最为明显。【结论】抑制NFκBp65和p38MAPK蛋白及磷酸化p38MAPK蛋白表达可能是上述不同治法方药抗脂肪肝作用的机制之一。 【关键词】? 脂肪肝／中药疗法；肝组织／病理学；疏肝；健脾；疾病模型，动物；大鼠 　　Effect of Different Therapies on Hepatic Nuclear Factor κBp65 and Kupffer Cell p38MAPK Expression in Rats with Fatty Liver 　　Abstract:? Objective To investigate the effect of different therapies on hepatic nuclear factor κBp65 (NFκBp65) and Kuffer cell p38MAPK expression in rats with fatty liver. MethodsNinetyeight SD rats were randomized into 7 groups: normal group，model group，liversoothing group (receiving gavage of Chaihu Shugan Powder 32 g·kg-1·d-1)，spleenstrengthening group (receiving gavage of Shen Ling Baizhu Powder 100 g·kg-1·d-1)，dampnessrespelling group (receiving gavage of Pingwei Powder 35g·kg-1·d-1)，bloodactivating group (receiving gavage of Gexia Zhuyu Decoction 71 g·kg-1·d-1) and the combination group (receiving gavage of seltmade prescription 119 g·kg-1·d-1). Except the normal group，the rats in other groups were fed with highfat forage and wine to induce fatty liver. After treatment for 12 weeks，the rats were executed to obtain the liver. Immunohistochemical assay was used for the detection of hepatic NFκBp65 activity and hepatic pathological changes