铅污染对菱幼苗SOD、POD及叶细胞亚显微结构的影响 Effects of lead polluted water on activities of superoxide dismutase, peroxidase and ultrastructure in leaves of Trapa bicornis seedlings.pdfVIP
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铅污染对菱幼苗SOD、POD及叶细胞亚显微结构的影响 Effects of lead polluted water on activities of superoxide dismutase, peroxidase and ultrastructure in leaves of Trapa bicornis seedlings
中国环境科学2009,29(2):136-141 ChinaEnvironmentalScience
{几占=,
t口,-j
李大辉+(中国科学院生态环境研究中心.北京100085)
等解体消失;细胞核的染色质与核质遭到破坏,核膜破裂.同样处理条件的叶片组织总DNA电泳显示,DNA完全断裂旱现弥散状,提示Pb“
毒害所致细胞死亡有别于细胞程序性死亡.Pb“对菱的毒害足多途径综合作用的结果.
bicornis):超氧化物歧化酶;过氧化物酶;亚显微结构;DNA电泳
关键词:Pb“;菱(Trapa
中图分类号:X17I.5 文献标识码:A 文章编号:1000-6923(2009)02—0136—06
of of inleavesof
Effectslead wateronactivities andultrastructure
polluted superoxidedismutase,peroxidase
Eco—Environmental of
bicornis Da—hui’(ResearchCenterfor Sciences,ChineseSciences,
Trapa seedlings.LI Academy
Environmental
Beijing100085,China).ChinaScience,2009,29(2):136-141
Abstract:Tothelead onwater bicornis of dismutase
investigatetoxicity caltrop(TrapaOsbeck),activitiessuperoxide
inleavesof wi山differentconcentrationoflead
(SOD)andperoxidase(POD),ultrastructureseedlings,treated solution,
were SOD was stimulatedandtheninhibitedwith lead
analyzed.Theactivityinitially increasing
lead 5 and20 thesame asthatinSOD it
concentration(1
high mg/L me/L),PODactivitypresented tendency analysis,but
increased inlowerseriesoflead and10 intheleaf
continually concentration(5mg/Lme/L).Ultrastructuraldamage cells,
aeatedwith20 observedundertransmissionelectron totalDNAextractedfrom
lead,Was
mg/L microscopy.Moreover,the
theselead—treatedleavesshowedaremarkablesmear thatthecelldeathWas
byagarosegelelectrophoresis,indicating
distinctfromtheso-called cell
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