汽爆玉米秸秆脱毒及发酵丁醇工艺的实验研究.pdfVIP

第27 卷第4 期 高 校 化 学 工 程 学 报 No.4 Vol.27 2013 年 8 月 Journal of Chemical Engineering of Chinese Universities Aug. 2013 文章编号：1003-9015(2013)04-0637-06 汽爆玉米秸秆脱毒及发酵丁醇工艺的实验研究 申利英, 常 春, 刘利平 (郑州大学 化工与能源学院, 河南 郑州 450001) 摘 要：研究了不同脱毒方法对汽爆玉米秸秆发酵产丁醇的影响及发酵工艺。实验共考查了十种不同的脱毒工艺路线， 结果表明：汽爆玉米秸秆经水洗预处理脱毒后，经酶解、离心去除沉淀物，并补加营养物质是一条较佳的工艺路线。 在此基础上，进一步利用单因素实验和正交实验，考察了发酵温度、pH 值、接种量、菌种种龄、培养基组成、发酵时 间对丁醇发酵的影响，得到较优的丁醇发酵工艺条件为，发酵温度 37 ℃，pH7.0 ，接种量7%，菌种种龄27 h ，培养 基组成：酵母抽提物 1.0 gL1、KH PO 0.8 gL1、MgSO ·7H O 0.1 gL1、(NH ) SO 2.0 gL1、FeSO ·7H O 0.03 gL1 、 2 4 4 2 4 2 4 4 2 尿素2.0 gL1 ，发酵时间48 h 。在此优化条件下，发酵液中起始糖浓度为50 gL1 时，丁醇的发酵浓度达到了9.42 gL1 。 关键词：汽爆玉米秸秆；脱毒；丁醇；发酵 中图分类号：TQ920.6 文献标识码：A DOI ：10.3969/j.issn.1003-9015.2013.04.015 Study on Detoxification and Butanol Fermentation Process of Steam-Exploded Corn Stalk SHEN Li-ying, CHANG Chun, LIU Li-ping (School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China) Abstract: The effects of different detoxification methods on the fermentation process and butanol production of steam-exploded corn stalk were studied. Ten different detoxification processes were investigated respectively, the results show that the suitable method of detoxification is rinsing the steam-exploded corn stalk firstly, then enzymatic hydrolysis and removing the sediments, followed by adding nutrients finally. On this basis, the effects of process parameters, such as fermentation temperature, pH value, inocul