电针对糖尿病胃轻瘫模型大鼠胃电及胃窦ghrelin的影响.pdfVIP

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电针对糖尿病胃轻瘫模型大鼠胃电及胃窦ghrelin的影响.pdf

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Chen HJ, Lin YP, Yang JW, et al. Effect of electroacupuncture on electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis. J Acupunct Tuina Sci, 2017, 15(4): 242-249 DOI: 10.1007/s11726-017-1008-9 Special Topic for 973 Program Effect of electroacupuncture on electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis 电针对糖尿病胃轻瘫模型大鼠胃电及胃窦ghrelin 的影响 Chen Hai-jiao (陈海交), Lin Ya-ping (林亚平), Yang Jian-wen (杨建文), Zhang Cheng-cheng (张程程), Peng Yan (彭艳), Liu Wei-wei (刘薇薇), Liu Li (刘丽), Liu Mi (刘密), Yue Zeng-hui (岳增辉) Hunan University of Chinese Medicine, Changsha 410208, China Abstract Objective: To observe the effect of electroacupuncture (EA) on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis (DGP). Methods: Fifty Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, 10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin (STZ), combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/(kg·bw)] by gavage. Rat’s blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by B