Core Substructure in Cyanobacterial Phycobilisomes英文文献.pdfVIP

Journal of Cellular Biochemistry 22:l-14 (1983) Biosynthesis of the Photosynthetic Apparatus: Molecular Biology, Development and Regulation 1-14 Core Substructure in Cyanobacterial Phycobilisomes Jeffrey C. Gingrich, DanielJ. Lundell, and Alexander N. Glazer Department of Microbiology and Immunology, University of California, Berkeley, California 94 720 The tricylindrical core of Synechocystis 6701 phycobilisomes is made u of four types of allophycocyanin-containingcomplexes: A, (aAP B, (aKpflAP)3 .]OK; C, (afPBafPfltP).1OK; D, (aAP 18.5K.99K; where AP is allophy- cocyanin, APB is allophycocyanin B, and ]OK, 18.5K, and 99K are polypeptides of 10,000, 18,500, and 99,000 daltons, respectively. The 18.5K polypeptide is a hitherto unrecognized biliprotein subunit with a single phycocyanobilin prosthetic group. The tricylindrical core is made up of 12 subcomplexes in the molar ratio of A:B:C:D: of 4:4:2:2. Complexes C and D act as terminal energy acceptors. From these results and previous analysis of the bicylindrical core of Synechococ- cus 6301 phycobilisomes [ 14,151 it is proposed that the two cylinders of the Synechocystis 6701 core, proximal to the thylakoid membrane, each have the composition ABCD, and that the distal cylinder has the composition A2B2. Key words: cyanobacteria, phycobilisome substructure, allophycocyanin complexes, biliproteins, energy transfer In cyanobacteria and red algae, phycobiliproteins are the major light-harvesting components of photosystem 11. These intensely colored chromoproteins