Production and characterization of two hemolysins of Bacillus cereus英文文献.pdfVIP

Canadian Journal Journal of Microbiology de microbiologie Prrblisl~edby PrrbliB por T H E N A T I O N A L RESEARCH COUNCIL OF C A N A D A LE CONSEIL N A T I O N A L DE RECHERCHES DU C A N A D A Volume 24 Number 1 1 November 1978 Volume 24 numero 1 1 novembre 1978 Production and characterization of two hemolysins of Bacillus cereus JAMESC. COOLBAUGH~A N D ROBERTP. WILLIAMS* Depnrtti~er~tof Microbiology titit1 It~~t~~rrttologl?,Bnylor College of Meclicir~e,Hortstoti, TX, U.S.A.77030 Accepted July 26, 1978 COOLBAUGH,J. C.. and R. P. WILLIAMS.1978. Production and characterization of two hemoly- sins of Bncillrrs errelis. Can. J. Microbiol. 24: 1289-1295. Bncilllrs ceretts strain B-48 produced two hemolysins with molecular weights of 52000 (H-I) and 31 000 (H-11). A mutant was isolated that produced only H-I1 but was identical with the wild type in all other respects. We exploited this mutant to produce H-I1 for study that was free of contamination by H-I. By manipulation of mediacomposition, we produced H-I in the absence of H-11. The hemolysins were precipitated differently by ammonium sulfate, and bothexhibited the Arrhenius effect when heated. Both hemolysins attached rapidly to erythrocytes; however, lysis by H-I was immediate, while lysis by H-I1 followed after a lag. HemoIysis by H-I and H-I1 increased in rate with increasing temperature