Reducible Polyoligo d arginine for Enhanced Gene Expression in Mouse Lung by Intratracheal Injection英文文献.pdfVIP

original article © The American Society of Gene Cell Therapy Reducible Poly(oligo-d-arginine) for Enhanced Gene Expression in Mouse Lung by Intratracheal Injection 1 1 1 1 Young-Wook Won , Hyun Ah Kim , Minhyung Lee and Yong-Hee Kim 1Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul, Republic of Korea Nonarginine (D-R9) has been reported to be one of the substitute to the polymer- or synthetic peptide–based gene deliv- most efficacious protein transduction domains (PTDs) for ery systems because they can enhance cellular uptake and nuclear the intracellular cargo delivery such as DNA, RNA, pro- translocation followed by gene expression.3 teins, and particles. Although oligoarginines are capable PTDs have the ability to effectively transduce bioactive car- of forming polyplex with DNA by electrostatic interaction, goes such as drug, protein, gene, and particle through cellular the length of oligoarginine can affect the toxicity and membrane.4 TAT peptide, Penetratin, transportan, and polyargi- gene expression. The reducible poly(oligo-d-arginine) nine are the most well-known PTDs. Using the TAT peptide, one (rPOA) composed of the Cys-(D-R9)-Cys repeating unit of the most well-characterized PTDs, the high transduction effi- forming disulfide bonds between terminal cysteinyl-thiol ciency of the various cargoes was achieved. Compared to the TAT groups of short peptides was hypothesized to show effi- peptide, L-9-arginines (L-R9) a