A clinically relevant orthotopic xenograft model of ependymoma that maintains the genomic signature of the primary tumor and preserves cancer stem cells in vivo英文文献.pdfVIP

Neuro-Oncology Advance Access published February 8, 2010 Neuro-Oncology doi:10.1093/neuonc/nop056 NEURO-ONCOLOGY A clinically relevant orthotopic xenograft model of ependymoma that maintains the genomic signature of the primary tumor and preserves cancer stem cells in vivo Litian Yu, Patricia A. Baxter, Horatiu Voicu, Sivashankarappa Gurusiddappa, Yijue Zhao, Adekunle Adesina, Tsz-Kwong Man, Qin Shu, Yu-Jing Zhang, Xiu-Mei Zhao, Jack M. Su, Lazlo Perlaky, Robert Dauser, Murali Chintagumpala, Ching C. Lau, Susan M. Blaney, Pulivarthi H. Rao, Hon-chiu Eastwood Leung, and Xiao-Nan Li Laboratory of Molecular Neuro-Oncology (L.Y., Q.S., Y.-J.Z., X.-M.Z., X.-N.L.), Texas Children’s Cancer Center (L.Y., P.A.B., H.V., S.G., Y.Z., T.-K.M., Q.S., Y.-J.Z., X.-M.Z., J.M.S., L.P., M.C., C.C.L., S.M.B., P.H.R., H.E.L., X.-N.L.), Department of Pathology (A.A.), Department of Neurosurgery (R.D.), Department of Molecular and Cellular Biology (H.E.L.), and Dan L. Duncan Cancer Center, Texas Children’s Hospital, Baylor College of Medicine, Houston, Texas (H.E.L.). Limited availability of in vitro and in vivo model systems population (2.2%) of CD1331 stem cells that can has hampered efforts to understand tumor biology and form neurospheres and display multipotent capabilities test novel therapies for ependymoma, the third most in vitro. The permanent cell line (BXD-1425EPN), common malignant brain tumor that occurs in children. which was derived from a passage II xenograft tumor To develop clinically relevant animal models of ependy- and has been passaged in vitro more than 70 times, moma, we directly injected a fresh surgical specimen expressed similar differentiation markers of the xeno- from a 9-year-old patient into the right cerebrum of graft tumors, maintaine