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Adv Biochem Engin/Biotechnol (2007) 108: 237–261
DOI 10.1007/10_2007_068
© Springer-Verlag Berlin Heidelberg
Published online: 31 July 2007
Development of Ethanologenic Bacteria
L. R. Jarboe1,2 () · T. B. Grabar1 · L. P. Yomano1 · K. T. Shanmugan1 ·
L. O. Ingram1
1Department of Microbiology and Cell Science, University of Florida,
Gainesville, FL 32611, USA
Jarboe@UFL.edu
2 Department of Chemical and Biological Engineering, Iowa State University,
Ames, IA 50011, USA
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 238
2 Engineering and Performance of Ethanologenic E. coli . . . . . . . . . . . 240
2.1 Ethanologenic Biocatalysts KO11 and LY01 . . . . . . . . . . . . . . . . . . 240
2.1.1 Engineering Scheme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 240
2.1.2 Utilized Substrates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242
2.1.3 Limitations and Challenges . . . . . . . . . . . . . . . . . . . . . . . . . . . 243
2.2 Ethanologenic Biocatalyst, Strain LY168 . . . . . . . . . . . . . . . . . . . . 243
2.2.1 Conversion of SZ110 to LY168 . . . . . . . . . . . . . . . . . . . . . . . . . 244
2.2.2 Ethanol Production by LY168 . . . . . . . . . . . . . . . . . . . . . . . . . . 244
2.3 Other Recombinant Ethanologenic E. coli Strains . . . . . . . . . . . . . . . 245
2.4 Non-recombinant Ethanologenic E. coli . . . . . . . . . . . . . . . . . . . . 246
2.5 Ethanol Production in Organisms Other than E. coli . . . . . . . . . . . . . 246
3 Metabolic an
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