Production of heterologous proteins in plants Strategies for optimal expression英文文献.pdfVIP

Biotechnology Advances 28 (2010) 427–435 Contents lists available at ScienceDirect Biotechnology Advances j ournal homepage: www. elsevier. com/ locate/ biotechadv Research review paper Production of heterologous proteins in plants: Strategies for optimal expression Priti N Desai, Neeta Shrivastava, Harish Padh ⁎ B. V. Patel Pharmaceutical Education and Research Development Centre, Ahmedabad, India a r t i c l e i n f o a b s t r a c t Article history: Plants are a promising expression system for the production of heterologous proteins, especially therapeutic Received 3 September 2009 proteins. Currently the majority of therapeutic proteins are produced in mammalian cell lines or bacteria. In Received in revised form 1 January 2010 a few cases insects, yeast and fungi have been developed for production of human proteins. However, these Accepted 25 January 2010 expression systems have limitations in terms of suitability, cost, scalability, purification and post- Available online 10 February 2010 translational modifications. Therefore, alternative expression systems are being developed in transgenic animals and transgenic plants. Transgenic plants could provide an attractive alternative in terms of low Keywords: Heterologous proteins p