Simulated microgravity perturbs actin polymerization to promote nitric oxide associated migration in human immortalized Eahy926 cells英文文献.pdfVIP

Protoplasma (2010) 242:3– 12 DOI 10.1007/s00709-010-0114-z ORIGINAL ARTICLE Simulated microgravity perturbs actin polymerization to promote nitric oxide-associated migration in human immortalized Eahy926 cells Jamila H. Siamwala S. Himabindu Reddy Syamantak Majumder Gopi Krishna Kolluru Ajit Muley Swaraj Sinha Suvro Chatterjee Received: 16 November 2009 /Accepted: 18 January 2010 /Published online: 20 February 2010 # Springer-Verlag 2010 Abstract Microgravity causes endothelium dysfunctions was not abrogated by phosphoinositol 3 kinase inhibitor and vascular endothelium remodeling in astronauts return- (Wortmanin). Bradykinin, an inducer of nitric oxide, promp- ing from space flight. Cardiovascular deconditioning occurs ted two folds higher nitric oxide production along with as a consequence of an adaptive response to microgravity simulated microgravity in a synergistic manner. We suggest partially due to the effects exerted at cellular level. that limited exposure to simulated microgravity increases Directional migration of endothelial cell which are central Eahy926 cell migration by modulating actin and releasing in maintaining the structural integrity of vascular walls is nitric oxide. regulated by chemotactic, haptotactic, and mechanotactic stimuli which are essential for vasculogenesis. We explored Keywords Simulated microgravity . Endothelial cells . the migration property of transformed endothelial cells Actin . Nitric oxide . Migration (EC) exposed to 2-h microgravity, simulated using a three- dimensional clinostat constructed based on blueprint pub- lished by the Fokker Space, Netherlands. Migration of EC Introduction was measured using the scrap wound healing in the presence or absence of actin polymerization