Prokaryotic whole transcriptome analysis deep sequencing and tiling arrays英文文献.pdfVIP

Microbial Biotechnology (2010) 3(2), 125–130 doi:10.1111/j.1751-7915.2010.00166.x Genomics update Prokaryotic whole-transcriptome analysis: deep sequencing and tiling arrays mbt_166 125..130 Roland J. Siezen,1,2,3,4* Greer Wilson2,5 and (Waters and Storz, 2009). To overcome the remaining Tilman Todt4,6 limitations of microarrays, a totally new approach to 1Kluyver Centre for Genomics of Industrial whole-transcriptome analysis was needed – and a much- Fermentation, 2600GA Delft, The Netherlands. awaited breakthrough in DNA sequencing came to the 2 TI Food and Nutrition, 6700AN Wageningen, The rescue. Here, we describe the first whole-transcriptome Netherlands. applications in prokaryotes and discover that a new trea- 3 NIZO food research, 6710BA Ede, The Netherlands. sure chest of regulation in prokaryotes is being opened. 4Center for Molecular and Biomolecular Informatics, Radboud University Nijmegen Medical Centre, 6500HB Whole-transcriptome sequencing Nijmegen, The Netherlands. 5 Science Consultant, Bowlespark 30, 6701DS With the dawn of next generation (or deep) sequencing Wageningen, The Netherlands. technologies in recent years (Ansorge, 2009; Metzker, 6 HAN University of Applied Sciences, Institute of Applied 2010), their application to high-depth sequencing of whole