Immune protection in carp, Cyprinus carpio L., after immunization with Aeromonas hydrophila crude lipopolysaccharide英文文献.pdfVIP

Journal ofFish Diseases 1988, 11, 237-244 Immune protection in carp, Cyprinus carpio L., after immunization with Aeromonas hydrophila crude lipopolysaccharide T. BABA , J . IMAMURA K. IZAWA Department of Veterinary Microbiology. University of Osaka Prefecture, Sakai, Osaka, Japan K. IKEDA National Research Institute of Aquaculture, Tamaki, Mie, Japan Abstract. Vaecination with crude lipopolysaccharide (LPS) induced better protee- tion against infection with Aeromonas hydrophila in carp than vaccination with formalin killed vaccine. Dipping fish in vaccine for 2h at 25X was more effective than intraperitoneal injection of the vaccine in procedural simphcity, lower stress loading and the degree of protection acquired. In carp immunized with crude LPS by the dip method, antibodies were not detected by bacterial agglutination, passive haemaggiutination and the agar diffusion tests. The results indicate that the proteetion against A. hydrophila infeetion in carp is not dependent on humoral immunity. Introduction Existing immunological studies in carp have mostly been based on antibody production . There is little information on protective immunity against infection with pathogenic microorganisms (Takahashi Kusuda 1971; Avtaiion Shahrabani 1975) in contrast to the situation in other groups of fish where there have been numerous studies on the nature of vaccines and the methods of vaccination. Antigens employed in protective immunity have consisted mainly