High Yield Growth of E.coZi at Different Temperatures in a Bench Scale Fermentor英文文献.pdfVIP

BIOTECHNOLOGY AND BIOENGINEERING, VOL. XVII, PAGES 227-239 (1975) High-Yield Growth of E.coZi at Different Temperatures in a Bench Scale Fermentor J. SHILOACH and S. BAUER, Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel Summary E. coli was grown exponentially a t different temperatures in a bench scale fermentor. pH was maintained at 6.8 by ammonia which served also as the nitrogen source. Glucose was introduced semi-continuously at a predetermined rate which ensured a glucose concentration of 25-50 g/liter during growth. The culture was sparged with pure oxygen. Yield constants for glucose, nitrogen, phosphorus, and oxygen were determined at the different temperatures of propagation. When all growt,h conditions, except temperature, were kept constant, the maximal possible yield of exponentially grown cell mass was found to be directly proportional to the doubling time. Concentrations of up to 55 g dry cells/liter culture were achieved. INTRODUCTION Maximal concentrations of bacterial cell mass under controlled culture conditions in fermentors are reported in the literature to be up to about 10 g dry cells/liter.1.2 In our previous communication3 we reported the investigation of the oxygen supply to the culture as a factor which limits the growth of aerobic organisms to this concen- tration. A correlation was shown between oxygen supply rates and the corresponding maximal exponential growth rates in a culture of E. coli in a bench scale fermentor. The maximal exponential growth rate, p, was defined by the relation p = yokekr (1) where