MCF 7 A HUMAN BREAST CANCER CELL LINE WITH ESTROGEN, ANDROGEN, PROGESTERONE, AND GLUCOCORTICOID RECEPTORS英文文献.pdfVIP

785 MCF-7: A HUMAN BREAST CANCER CELL LINE WITH ESTROGEN,ANDROGEN, PROGESTERONE,AND GLUCOCORTICOIDRECEPTORS K.B, Horwitz, M.E. Costlow, and W.L. McGuire Departmentof Medicine University of Texas Health Science Center 7703 Floyd Curl Drive San Antonio, Texas 78284 .,eceivecl:g/24-/73 ABSTRACT We have identified receptors for glucocorticoids,progestins, and androgens in a human breast tumor cell line (MCF-7)known to have estrogen receptor. Sucrose density gradients show that MCF-7 cytosol contains approximately 100 fm/mg protein estradiol (E2-3H) receptor,more than 300 fm/mg protein progesterone receptor (measured with R5020-3H), about 40 fm/mg protein Sa-dihydrotestosterone (S~X-DHT-~H)receptor, and 800 fm/mg glucocorticoidreceptor (measured with dexamethasone-3H). D association constants obtained by Scatchard analyseswere approximately0.6 x 10-lM (E2),1 x 10egM (RSOZO), 2.8 x lo-1OM (Sa-DHT)and 8 x lo-SM (dexamethasone).No cross competi- tion was found for estrogen receptor,but progestins competed for androgen and glucocorticoidbinding. The androgen,but not the glucocorticoid,partially competed for R5020 binding to progesterone receptor. This first demonstrationof 4 classes of steroid receptors in human breast cancer means that MCF-7 may be an excellent in vitro model for studying the mechanism of tumor response to endocr=e- therapy as well as the complex relationshipsbetween binding and biological actions of these hormones. INTRODUCTION Although 55 to 60% of estrogen receptor containing (ER+)human breast tumors respond to endocrine ablative or additive therapy, the growth of 40% of ER+ tumors is unaffectedb