Head to tail fusions of camelid antibodies can be expressed in planta and bind in rumen fluid英文文献.pdfVIP
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Biotechnol. Appl. Biochem. (2009) 53, 111–122 (Printed in Great Britain) doi:10.1042/B 111
Head-to-tail fusions of camelid antibodies can be expressed
in planta and bind in rumen fluid
y
r
t
Somrutai Winichayakul*, Anton Pernthaner†, Richard Scott*, Ruth Vlaming* s
and Nick Roberts* 1 i
*Forage Biotechnology, AgResearch Grasslands, Tennent Drive, Palmerston North, New Zealand, and †Hopkirk Institute, m
Animal Health, AgResearch, Massey University Campus, Palmerston North, New Zealand e
h
We have compared the accumulation of recombinant high conformational stability with a reversible unfolding c
variable heavy-chain portions [VHH (variable heavy- behaviour after prolonged incubation at high temperature o
chain antibody from camelids)] of camelid antibodies [6] and are stable and active under harsh conditions, such as i
in a variety of subcellular compartments produced in detergents [7] and the digestive tract (for a review, see [8]). B
planta. The
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