[生物学]液相质谱技术LC-MS的应用.pdf

液相质谱技术(LC-MS)的应用 熊 英 2009年11月26 日 Multiple Dimension LC-MS Thermo-Fishers, Linear Ion Trap MS (LTQ) linear ion Trap MS, LTQ auto-sampler capillary LC separation system (located in Life Science Building, Room 338) Bottom-Up Top-Down Approaches N C linear protein proteolysis peptide ions “Bottom-Up” Approach Representative paper: N.L Kelleher et al. “Top down versus bottom up protein characterization by tandem high-resolution mass spectrometry.” JACS, 121, 806-12, 1999 computing system LC system mass spectrometer ion source Separation System C18 reversed phase LC Sol A: 0.1 % Formic acid in ddH2O; 300 Å, 5 µm Sol B: 0.1 % Formic acid in acetonitril; 75 µm X 150 mm (I.D. X L) Gradient: 15-65 % acetonitrile. Link AJ, Eng J, Schieltz DM, Carmack E, Mize GJ, Morris DR, Garvik BM, Yates JR 3rd. Direct analysis of protein complexes using mass spectrometry. Nat Biotechnol. 1999 Jul;17(7):676-82 protein extract digest “peptidome”