香菇生长发育过程中漆酶基因家族的转录表达研究.docxVIP

香菇生长发育过程中漆酶基因家族的转录表达研究*秦澎1李津1辜运富1曾先富2向泉桔1**1四川农业大学资源学院微生物系四川成都 6111302成都市农林科学院园艺研究所四川成都 611130摘要香菇依靠分泌胞外漆酶降解木质素作为自身营养物质，其在香菇的营养代谢和生长发育过程中起着促进作用。为探究漆酶基因在香菇生长发育过程中的作用，本实验采用RT-qPCR方法分析10个漆酶基因在香菇4个不同发育阶段（菌丝体、原基、幼小子实体和成熟子实体）的转录表达情况。结果显示，不同漆酶基因在香菇不同发育阶段的转录表达存在差异，其中5个漆酶基因（Lelac1、Lelac3、Lelac5、Lelac8和Lelac9）的转录表达表现出差异性和发育阶段特异性，另外5个漆酶基因（Lelac2、Lelac4、Lelac7、Lelac10和Lelac11）只表现出差异性。lacc2和lacc3随着香菇的生长，其转录表达量持续上升，可能与成菇过程有关；lacc1和lacc7在菌丝体阶段大量表达，可能参与菌丝体的生长代谢；lacc8在原基时期表达量显著高于其它时期，与原基分化有关。关键词香菇；漆酶；菌丝体；原基；子实体；RT-qPCRTranscriptional expression profiles of laccase gene family in different developmentstages of LentinusedodesQINPeng1LI Jin1 GU Yunfu1 ZENG Xianfu2 XIANG Quanju1**1 Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, Sichuan 611130, China2 Institute of Horticulture, Chengdu Academy of Agricultural and Forestry Sciences, Chengdu, Sichuan 611130, ChinaAbstractObjectives: Laccase plays an important role in nutrition metabolism and growth of Lentinusedodes. In order to explorethe role of laccasegene family in the growth and developmentof L.edodes, the transcriptional expression profilesof laccase genes in different development stages of L.edodesare needed to be studied.Methods: Transcriptional expression profiles of 10 laccase genes in four different development stages: mycelium,primordium, young fruiting body and mature fruiting body, were analyzed by RT-qPCR.Results:The results showed that differentlaccase genes exhibit specific ordifferential expression profiles across the development stages. Five laccase genes（Lelac1、Lelac3、Lelac5、Lelac8andLelac9）showed specific ordifferential expression profiles, while the other five laccase genes（Lelac2、Lelac4、Lelac7、Lelac10 andLelac11）showeddifferential expression profiles.The relative expression levels of lacc2 and lacc3 increased with the growth and development,implying their participationsin the fruiting process. Lacc1 and lacc7 are highly expressed in mycelium stage, suggestingthat they fu