慢病毒载体的构建PPT.ppt

慢病毒载体的构建 －－Gateway to lentiviral vector;Introduction ; Lentiviruses life cycle;Lentiviral Vector SystemsUnder Development;The outline of this ppt;一 HIV-1 life cycle ;二 HIV-1病毒颗粒结构;三 载体系统构建的基本原理 ;四 载体系统的设计;Components of the lentiviral system;五 包装系统的设计 ----- HIV-1-Derived lentiviral vector production ,concentration titration;1. HIV-1-Derived lentiviral vector production titration;2.HIV-1 Vector Production Supernatant recoveries concentration;六 The difference of Packaging Cells for LVV with other retroviruses ;七 The Development of LV VectorsThe 1st Generation;The Development of LV VectorsThe 2nd Generation;The Development of LV VectorsThe 3rd Generation;八 Advantages and Disadvantages of Lentiviral vectors;Advantages and Disadvantages of Lentiviral vectors;九The comparison of Lentiviral vectors with other vectors;References;8. Wong, L. F., et al. (2004). Transduction patterns of pseudotyped lentiviral vectors in the nervous system. Mol. Ther. 9: 101 – 111. 9. Wiznerowicz, M., and Trono, D. (2005). Harnessing HIV for therapy, basic research and biotechnology. Trends Biotechnol. 23: 42 – 47. 10. Consiglio, A., et al. (2004). Robust in vivo gene transfer into adult mammalian neural stem cells by lentiviral vectors. Proc. Natl. Acad. Sci. USA 101: 14835 – 14840. 11. Jakobsson, J., Ericson, C., Jansson, M., Bjork, E., and Lundberg, C. (2003). Targeted transgene expression in rat brain using lentiviral vectors. J. Neurosci. Res. 73: 876 – 885. 12. Miletic, H., et al. (2004). Selective transduction of malignant glioma by lentiviral vectors pseudotyped with lymphocytic choriomeningitis virus glycoproteins. Hum. Gene Ther. 15: 1091 – 1100. ;