不同pH值酿酒酵母分批发酵生产谷胱甘肽数学模型建立-食品科学.PDFVIP

162 2013, Vol.34, No.13 食品科学 ※生物工程 不同pH值酿酒酵母分批发酵生产谷胱甘肽 数学模型建立 1,2 1 1,2 1,* 郑丽雪 ，谢群凡，王立梅 ，齐 斌 (1.常熟理工学院生物与食品工程学院，江苏 常熟  215500 ；2.苏州市食品生物技术重点实验室，江苏 常熟  215500) 摘  要：以麦芽汁为发酵培养基，考察不同pH值条件下酿酒酵母CS105 15-1分批发酵合成谷胱甘肽的影响，对实验 数据进行分析并拟合出数学模型。结果表明：两种pH值条件下高斯拟合对谷胱甘肽产量数据的拟合效果较好，相 关系数(R2)分别为0.99235(pH5.0) 、0.98842(pH6.0) ，而对生物量、基质消耗拟合效果差。另外，当发酵pH值恒定在 5.0时，32h 时谷胱甘肽产量达最大，为72mg/L ，此时生物量为4.59g/L ；当发酵pH值恒定为6.0时，同样也是32h 时谷 胱甘肽产量达最大，为91mg/L ，此时生物量为4.11g/L 。 关键词：谷胱甘肽；麦芽汁；酿酒酵母；高斯拟合 Mathematical Modeling of Glutathione Production by Saccharomyces cerevisiae in Batch Fermentation at Different pH 1,2 1 1,2 1,* ZHENG Li-xue ，XIE Qun-fan ，WANG Li-mei ，QI Bin (1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China ； 2. Suzhou Key Laboratory of Food and Biotechnology, Changshu 215500, China) Abstract ：A mathematical model of glutathione production by Saccharomyces cerevisiae Y5 18 in batch fermentation at different pH was established with malt sprout extract as the fermentation medium. The results showed that Gaussian fitting model was in good agreement with the experimental observations for glutathione production at different pH, with correlation coefficient (R2) of 0.99235 (pH5.0) and 0.98842 (pH6.0), but not suitable for biomass and substrate consumption prediction. Glutathione production reached the maximum of 72 mg/L at 32 h yielding a biomass of 4.59 g/L when pH was kept constant at 5.0. In contrast, maximum glutathione production of 91 mg/L was also obtained at 32 h along with a biomass of 4.11 g/L when pH was kept constant at 6.0. Ke