铝胁迫下钙对小麦根液泡膜功能和膜脂组成的影响南京农业大学学报.PDFVIP

南京农业大学学报 　2000 , 23 ( 1) : 10～13 　 　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　 Journal of Nanjing Agricultural University 铝胁迫下钙对小麦根液泡膜功能和膜脂组成的影响 何龙飞 　沈振国 　刘友良 (南京农业大学农学系 , 南京 210095) 摘要 　用不连续蔗糖密度梯度离心法分离小麦根尖液泡膜微囊 , 比较了铝胁迫下钙对耐铝小麦品种Altas 66 和铝敏感品 种 Scout 66 的根长、根系液泡膜ATP 酶和焦磷酸酶活性、膜脂组成的影响。铝胁迫下增加营养液中的钙浓度 , 可明显缓 解铝对两品种根伸长的抑制作用 , 提高根系液泡膜 H+ ATP 酶、焦磷酸酶和 Ca2 + ATP 酶活性 ; 增加磷脂含量 , 部分抑制 了铝胁迫所引起的半乳糖脂含量上升。 关键词 　铝胁迫 ; 钙 ; 小麦 ; 液泡膜 ; 膜脂 分类号 　S4351213 Effects of calcium on the function and lipid composition of tonoplast from two wheat ( Triticum aestivum L. ) cultivar roots under aluminum stress He Longfei , Shen Zhenguo and Liu Youliang (Dept of Agronomy , Nanjing Agric Univ , Nanjing 210095) ABSTRACT 　Tonoplast was isolated from root tips of two wheat ( Triticum aestivum L. ) cultivar roots , Altas 66 (Altolerant) and Scout 66 (Alsensitive) seedlings by discontinous sucrose density gradient centrifugation. Effects of calcium on the root length , ATPase and pyrophosphatase ( H+ PPase) activities , lipid compositions of tonoplast under Al stress were assayed. The increase of Ca concentration in + + the nutrient solution ameliorated distinctly the inhibition of Al on root growth , improved the activities of H ATPase , H PPase and 2 + Ca ATPase , increased the content of phospholipid , and decreased the content of galactose in lipids of tonoplast from two cultivar roots under Al stress. Key words 　alumimum stress ; calcium ; Triticum aestivum L. ; tonoplast ; lipid