[理学]MicrobiologyGrowth微生物学.pdf

Growth of bacteria and flora formation in human body Growth and cultivation of bacteria  Definition of growth: an orderly increase of all components of a cell  Genome replication  Speed of growth (Who say the last word ?) Essential nutrients for bacteria growth  Source of carbon  Source of nitrogen  Growth-supporting factors  Water (why ?) Cultivation of bacteria Carbon comes from: CO2 (autotrophs) Carbohydrates (heterotrophs) Growth curve of bacteria  How does it look like  Lag phase (why?)  Log phase (can we keep it long?)  Constant phase (why?)  Decline phase (source of trouble? growth curve Growth curve in Laboratory Groth curve with clinical significance Cultivation of bacteria Artificial medium for: Proliferation Differentiation Identification Cultivation of bacteria Nitrogen comes from: N2 (Autotrophs) Protein (Heterotrophs) Physical and chemical factors to support bacteria growth  Medium  Temperature  Humidity  Gas environment (aerobic and anaerobic)  pH condition  Other factors Cultivation of bacteria Artificial medium for: Proliferation Differentiation Identification Physical states clinical application  Solid medium: colony isolation  Semi-solid medium: motility  Liquid medium: proliferation Plate Method Biosafety cabinets - BSC Class I Class II Class III May 2009 Mats Spångberg 15 Culturing bacteria in vitro By her with these We may use smart technology if we are smart and if we have enough Molecular Approaches to Study the Intestinal Microbiota (Selective)