扫描电化学显微镜直接电沉积法构建葡萄糖氧化酶微米点Formationof.PDFVIP

2007 年第65 卷 化 学 学 报 Vol. 65, 2007 第23 期, 2750～2754 ACTA CHIMICA SINICA No. 23, 2750～2754 ·研究论文· 扫描电化学显微镜直接电沉积法构建葡萄糖氧化酶微米点 韩帅兵* 段艳林 李菲菲 ( 山东大学化学与化工学院 济南 250100) 摘要 利用自制的凹形电极在铂基底电极上直接构建了葡萄糖氧化酶微米点. 首先, 将电聚合和电化学刻蚀法相结合 制备了凹形铂微米电极. 然后将此种电极作为参比及辅助电极, 基底铂电极作为工作电极, 利用葡萄糖氧化酶在合适 的条件下(浓度、一定量Triton X-100 存在、电极电位等) 由于电极表面pH 的降低可以在铂电极上电沉积这一特性, 将 酶固定在铂基底电极上, 微修饰得到了具有活性的葡萄糖氧化酶微米点. 最终用扫描电子显微镜和扫描电化学显微镜 对所得微米点进行了表征. 所得微米点直径约20 μm, 且具有催化活性. 该方法简便, 干扰因素较少. 关键词 扫描电化学显微镜; 葡萄糖氧化酶; 凹形铂微米电极 Formation of Glucose Oxidase Microscopic Spots by Means of Electrodeposition with Scanning Electrochemical Microscopy HAN, Shuai-Bing* DUAN, Yan-Lin LI, Fei-Fei (School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100) Abstract Microscopic spots of glucose oxidase (GOx) were fabricated on platinum substrate electrode with a self-made concave platinum micrometer electrode. Firstly, the concave platinum micrometer elec- trodes were prepared by means of phenol-allylphenol copolymer insulation and electrochemical etching. Using concave platinum micrometer electrode as reference and counter electrodes and platinum substrate as a working electrode, GOx on the concave platinum electrode was then electrochemically deposited and fixed on a platinum substrate to form microscopic spots with biological activity by micro-decoration, based on the fact that GOx could be electrochemically deposited on