插入突变在水稻功能基因组学中的研究进展-生物技术通报.PDFVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2009 5 1, 2 1 苏红 印莉萍 ( 1 , 100048; 2 , 721013) : 构建饱和的基因突变体库是最直接有效的分析鉴定基因功能的方法根 插入突变源不同可分为TDNA 插 入突变转座子插入突变等主要介绍这两种方法的原理及其在水稻功能基因组学研究中的应用和进展, 并分析和讨论了插 入突变在水稻功能基因组学研究中存在的困难和发展趋势 : 插入突变 TDNA 转座子 水稻功能基因组学 Study on the InsertionalM u tagensis in R ice Functional G enom ics 1, 2 1 Su Hong Y in L ip ing 1 2 ( College of Lfi e Sciences, Cap italN ormal University, B eij ing 100048; Baoj i VocationalTechnology College, Baoj i 721013) Abstrac:t Inse tional m utagenesis has been known as a e liab le m ethod to const uct a ice mutant lib a yA cco ding to the sou ces of inse tional m utagenesis, the m utag enesis can be div ided into TDNA inse tiona l m utag enesis, t ansposon inse tion muta tionT his pape m a inly int oduced the p inciples of these two m ethods and its app lication in ice functiona l genom ics esea ch, especia l ly in ice func tiona l genom ics study K ey w ords: Inse tiona lmu tagensis TDNA T anspo son R ice functional genom ics , ; [ 3] , , ; [ 4 ] mRNA , , , , , , , , ,