大规模基因组测序的原理与方法演示课件文.ppt

Consensus Sequence Gap Mis-Assembly (Inverted) Shotgun Sequencing III: FINISHING Consensus Mis-Assembly (Inverted) Shotgun Sequencing III: FINISHING Shotgun Sequencing III: FINISHING High Accuracy Sequence: 1 error/ 10,000 bases Consed软件显示序列组装结果界面 1、Filling “intraclone gaps” BAC-----453F3’s finishing Sp6 T7 ? First 4 primers 1 2 3 4 All the contigs walked hundreds’ bps toward the gaps. 453F3’s 2600 reads 12 contigs Overlapping BAC-454F24’s 200 reads + Sp6 T7 1 3 2 4 a b c Second 3 primers 1200bp’s AT-rich , (CATATATA)n repeat. F