血管内皮细胞体外培养及生物学特性的探讨_庞文丽.pdfVIP

# 4 # 2003 2 ( 23 1 ) J Stomatol, Vol. 23, No1 1, Feb. 2003 1 1 2 庞文丽 潘明华 郭 军 [ ] , 012% , SP Ø , ( 1) , (2) , ( 3) , [ ] ; ; + [ ] Q 81311 1 [ ] A [ ] 1003-9872(2003) 01-0004-03 The Biologic Characteristics of HumanVein Endothelial Cells CulturedinVitro PANG Wenli, PAN Minghua, GUO Jun . (Department of Stomatology, Shizuishan irstHosp ital, Shizuishan 753200, China) [Abstract] Objective In view of t e similarity between endot elial cell of umbilical vein and endot elial cell of cavernous emangioma in biological c aracteristics, we ave separated t e endot elial cellsfrom umbilical vein and cultured in intro to distinguis t eir biological c arac- teristics, so t at we could establis t e base of drug intervention experiment of endot elial cells in vitro. Methods We obtained t e endot e- lial cells by enlymatic perfusion digestion of umbilical vein twice using trypsinase (012%) . We observed t e morp ologic c aracteristics of en- dot elial cells under t e inverted microscope and fuloroscopy, and indentified t e factor Ø antigen by SP immuno istoc emical met od. We draw cell growt curve by observing t e growt of endot elial cells cultured in vitro. Results ( 1) Wel-l purified HVEC wit few toxic effects were obtained by two-staged digestion of trypsinase. ( 2) HVEC in confluent primary culture reproduce t e epit elioid organization of t e vascu- lar lining in situ. ( 3) T e marked structure of junctional integrity and substratum attac ed to one side of cells may be a supplement to identify HVEC. Conclusion T e c aracteristics of t e cells under fuloroscopy can be used to identify HVEC. [ ey words] Endot elial cell; Cell culture; Biological c aracteristics ,