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i n t e r n a t i o n a l j o u r n a l o f h y d r o g e n e n e r g y 3 5 ( 2 0 1 0 ) 1 1 2 8 – 1 1 3 7 Available at www.sciencedire ct .com j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c om / l o c a t e / h e A quantitative analysis of hydrogen production efficiency of the extreme thermophile Caldicellulosiruptor owensensis OLT Ahmad A. Zeidan*, Ed W.J. van Niel Department of Applied Microbiology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden a r t i c l e i n f o a b s t r a c t Article history: Caldicellulosiruptor owensensis strain OLT (DSM 13100) is an obligately anaerobic, extreme Received 20 October 2009 thermophilic bacterium that is capable of utilizing a broad range of carbohydrates and Received in revised form producing H2 as a metabolic by-product. The performance of C. owensensis on glucose and 18 November 2009 xylose was analyzed in lab-scale bioreactors to assess its potential use in biohydrogen Accepted 21 November 2009 production. Acetate, H2, and CO2 were the main end products during exponential growth of Available online 16 December 2009 the organism on either sugar. Lactate production was triggered during the transition into the stationary phase and was associated with an increase in the levels of specific L-lactate Keywords: dehydrogenase activity. In addition, minor amounts of ethanol and propionate could be Biohydrogen detected. H2 and acetate yields were lower on xylose than on glucose, marking an opposite Extre