一株产吲哚菌的分离鉴定及其碳源发酵特性分析.pdfVIP

摇 南京农业大学学报摇 2011,34(3):140-142 摇 Journal of NanjingAgricultural University http:/ / 試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試 王政,毛胜勇,朱伟云.一株产吲哚菌的分离鉴定及其碳源发酵特性分析[J].南京农业大学学报,2011,34(3):140-142 一株产吲哚菌的分离鉴定及其碳源发酵特性分析 王政,毛胜勇,朱伟云* (南京农业大学消化道微生物研究室,江苏 南京210095) 摘要:利用含L-色氨酸的基础培养基从仔猪新鲜粪样中分离产吲哚的菌株,根据菌落形态、生理生化特征分析,鉴定该菌 株为Escherichia属,命名为wz_1977。 通过批次培养的方法,研究了wz_1977 以不同碳源为底物时的生长和吲哚生成情况。 结果表明:以葡萄糖为底物时,wz_1977 的菌落数目显著高于其他各组(P0.05),而吲哚生成水平最低;以半乳寡糖为底物 时,wz_1977 的菌落数目仅次于葡萄糖,而吲哚生成水平高于葡萄糖组;以果寡糖为底物时,wz_1977 的菌落数目与对照组 (不含碳源)相比没有显著差异(P0.05),而吲哚生成水平稍低于对照组(P0.05)。 结论:分离出1株产吲哚菌wz_1977, 该菌的发酵模式为:以葡萄糖为底物时以合成菌体蛋白为主,以果寡糖为底物时以生成吲哚为主。 关键词:分离;产吲哚菌;碳源利用 中图分类号:Q939.96摇 摇 摇 摇 文献标志码:A摇 摇 摇 摇 文章编号:1000-2030(2011)03-0140-03 Carbohydrate utilization of an indole鄄producing bacterium WANGZheng,MAO Sheng鄄yong,ZHU Wei鄄yun* (Laboratory of Gastrointestinal Microbiology,Nanjing Agricultural University,Nanjing210095,China) Abstract:An indole鄄producing bacterium namedwz_1977 was isolated from fresh faeces of piglets by enrichment in basal medium supplementedwithL鄄tryptophan,and the strain was identified asEscherichia sp.based on its morphology,biochemistry test and 16S rRNA sequencinganalysis.Wealsoinvestigateditscharacteristicsofthegrowthandindoleproductionwithglucose(GLU),galactool鄄 igosaccharides(GOS)andfructooligosaccharides(FOS)as substrate respectively.The results indicated that the viable counts did not differ between the control(no carbohydrate)and FOS group,whereas indole concentrations in FOS group were lower than those in control group(P0.05).With GOS as substrate the viable counts of strain wz_1977 were lower and indole levels were higher when compared to GLU group,in whichtheviablecountswerehighe