中药补骨脂的生药学研究.pdf

·138 ·                                    国际中医中药杂志2014年2月第36卷第2期 Int J Trad Chin Med, February 2014, Vol. 36, No.2  ·论著 · 中药补骨脂的生药学研究 梁丽娟 赵奎君 董婷霞 詹华强 【摘要】 目的 对常用药补骨脂进行生药学鉴别研究，为补骨脂药材的鉴定与开发利用提供科学 依据。方法 采用石蜡切片法和粉末制片法进行制片，对补骨脂横切面及粉未进行显微鉴别，同时进行 薄层色谱和理化鉴定。结果 对补骨脂的形态特征，果实组织横切面和果实粉末的显微特征进行了描述； 薄层色谱结果显示，补骨脂药材在与补骨脂素及异补骨脂素相同位置上有相同颜色的荧光斑点；十批药 材水分、灰分测定结果均符合药典规定标准，水浸出物及 70%乙醇浸出物含量分别为21.93%～39.68%， 22.03%～31.77% 。结论 本实验采用的方法，简单可行，结论可靠，可鉴别该药材。 【关键词】 补骨脂；显微鉴定；理化鉴定；薄层色谱 Pharmacgnosical study of Fructus Psoraleae Liang Lijuan *, Zhao Kuijun, Dong Tingxia, Zhan Huaqiang. *Beijing Friendship Hospital affiliated to Capital Medical University, Beijing, 100050, China 【Abstract 】 Objective To provide scientific evidence for the identification and development of fructus psoraleae through its pharmacognostical study. Methods To identify fructus psoraleae by paraffin section and powder method, meanwhile, morphological identification and physical and chemical identification were also adopted. Results The morphological and microscopic characteristics of transverse section and powder of fructus psoraleae were described; fluorescent speckles of the same color were found in the same place of test samples and psoralen and isopsoralen reference by thin layer chromatography; results of water and ash determination all complied with Chinese pharmacopoeia specification, water and 70% ethanol extractives were 21.93～39.68%，22.03～31.77%, respectively. Conclusion The established method was simple and practical with reliable results, and suitable for the identification of fructus psoraleae. 【Key words 】 Fructus psoraleae ；Pharmacognostic identification ；Physical and chemical identification ； Thin layer chromatography 补 骨 脂 为 豆 科 植 物 补 骨 脂 （ Psoralea 酮类（主要有补骨脂甲素 corylifolin、补