1999 Colloid Surf A-酶吸附.pdfVIP

Colloids and Surfaces A: Physiochemical and Engineering Aspects 153 1999 429 433 Adsorption of enzymes onto submicron-sized temperature-sensitive composite polymer particles and its activity 1 M. Okubo, H. Ahmad Department of Chemical Science and Engineering, Faculty of Engineering, Kobe Uniersity, Kobe 657-8501, Japan Abstract Temperature-sensitive composite polymer particles were prepared by seeded emulsion copolymerization of dimethylaminoethyl methacrylate and ethylene glycol dimethacrylate with 0.14-m-sized polystyrene seed particles. Its reliability as a carrier for biomolecules was evaluated by measuring the enzymatic activity of trypsin adsorbed on the composite particle at a temperature above the lower critical solution temperature LCST . Conformation of trypsin desorbed from the particle surface by lowering the temperature to below the LCST was examined with circular dichroism spectroscopy and compared with the native one. Both results indicate that enzymatic activity of the trypsin retained during the adsorptiondesorption measurement. 1999 Elsevier Science B.V. All rights reserved. Keywords: Temperature-sensitive; Morphology; Adsorption; Trypsin; Specific activity 1. Introduction EGDM with submicron-sized polystyrene PS seed particles 1 . The particle consisted of a PS Recently we reported on the preparation of a core