叶绿体蛋白质组研究进展.pdfVIP

, 2008, 2 8( 1) : 0 194- 0203 A cta Bot. Bor eal. -Occid ent . Sin. : 1000- 4025( 200 8) 01- 0194- 10 * 彭 浩, 林文芳, 朱学艺* ( , 36 1005) : , , , , , : ; ; ; ; : Q 75 : A Research Progress in Chloroplast Proteome * P EN G H ao, L IN Wen- ang, ZH U Xue-yi ( School o Li e S cien ces, Xiamen U nivers it y , X iamen , Fu jian 36 1005, China) Abstract: Subcellular pro teom ics is curr ent ly t he most e ect ive appr oach t o char act eriz e subcel lular com- part ment s . Based on the pow er ul com binat ion o subcellular ractionat io n w it h prot eomic analysis, it makes possible t o char act eriz e all chlo roplast prot eins co mplet ely. Chlor oplast pr ot eo me is one o the most inten- sive aspect s o the plant subcellular prot eo mics resear ch . By using subcellular prot eome t echnique, more and more choloplast ic pr ot eins have been ident i ied . T he present rev iew sum marizes t he recent pro gress in pro- t eins locat ed in the di erent chlor oplastic com part ments , including t he inner and out er envelop membranes, chloro plast st rom a, t hylako id m em branes and t hylakoid lum en . We hope it cont ribut es t o understand the charact er s o chlor opl ast ic prot eins and t heir unct ions in the chloroplast ic metabolism netw o rks . Key words: chloro plast ; t hylakoid; subcellular prot eo me; t w o- dimension electr ophoresis ( 2- DE ) ; mass spec- t ro metr y , ( t w o- dimensional g el elec- , t ro phoresis , 2- DE) , , , , perco ll , / 0 ( subpro teom e) , 2-DE * : 2007- 0-0; : 2007- 12- 14 : ( 30470 164) ; ( N CET XM U ) : ( 19