医用臭氧对大鼠骨骼肌病理学的影响英文摘要.doc

医用臭氧对大鼠骨骼肌病理学的影响英文摘要： Impact of Different Concertrations of Ozone Injection on Pathological Changes of Rat Skeleta Muscle 　 [Abstract]　Objective　 To evaluated the pathological changes of skeletalmuscle in normal rabbit by different concentrations of ozone injection. Methods　30 wistar rars were randomly divided into 5 groups (n=6). group Ⅰozone10， group Ⅱozone30， group Ⅲozone50， group Ⅳozone80， group Ⅵcontrol. In group Ⅰ， Ⅱ， Ⅲand Ⅳ，1ml of ozone 10μg/ml、30μg/ml、50μg/ml、80μg/ml was injected into right ectogluteus respectively，and in group Ⅴ，punctura at the same point without any injection. Biopsy was performed after 10min，30min and 3 weeks with the lightmicroscope，selecting the injection area. Results Compared to the control, light microscope of group Ⅱ, Ⅲ and Ⅳ showed myocyte swelling, myocyte′s bouncary became unclear, transverse striation became disorder, myocyte necrosis, dissolve and vacuolus degeneration partly after 10min both in two groups. Inflammation cells infiltrated in muscle interspace and blood vessel based on myocyte necrosis and dissolve in the injection area after 30min. Myocyte atrophy obviously, fibrous tissue accrementition ponderosus, hyalinization partly could be seen after 3 weeks. There were no abnormal pathological changes in group Ⅰand Ⅴ. Conclusions　 Myocyte damage occurs after injection of ozone with concentration of 30μg/ml、50μg/mland 80μg/ml， fibrous tissue repairs the damage after 3 weeks. Ozone with concentration of 10μg/mldo little harm to skeletal musle. [Key words] Medical Ozone; Skeletal Muscle; Pathology；　 中文摘要： 　　【摘要】　目的　通过观察医用臭氧注射后，不同时间段大鼠臀大肌结构的变化，来评价不同浓度医用臭氧在骨骼肌注射中的安全性。方法　30只大鼠随机分5组（n=6），医用臭氧组：臭氧10、臭氧30、臭氧50、臭氧80组分别注射浓度为10μg/ml、30μg/ml、50μg/ml、80μg/ml的医用臭氧1ml，对照组仅行穿刺不注射；穿刺注射部位在右侧臀大肌，注射的气体均为1ml。注射后10min、30min及3周后分别对医用臭氧注射点取活检在光镜下行病理学检查。结果 与对照组比较，光镜下显示臭氧30、臭氧50及臭氧80三组注射医用臭氧后10min注射点见肌细胞肿胀，肌细胞边界不清，部分肌细胞坏死溶解，空泡变性；30min后在肌细胞坏死溶解的基础上出现了肌细胞间隙和血管内炎症细胞浸润；3周后切片显示肌细胞明显萎缩，较多纤维组织增生，部分伴有玻璃样变，而臭氧10组与对照组间比较没有明显差异。结论　浓度为30μg/ml、50μg/ml、80μg/ml