创新导管固定法用于构建硬膜外阻滞动脉模型.doc

改良导管固定法在硬膜外阻滞大鼠模型构建中的应用研究 周波1，陈国忠1，3，刘小龙1，许秀艺2，刘韧3，王丽萍3 (1. 福建医科大学福总临床医学院麻醉科，福州，350025；2. 厦门市妇幼保健院麻醉科，厦门，361003；3. 南京军区福州总医院麻醉科，福州，350025) 【摘要】 目的：探讨改良硬膜外导管固定法应用于构建硬膜外阻滞动物模型的可行性及效果。方法：采用随机数字法将24只雄性Wistar大鼠分为实验组和对照组，每组12只，用于构建硬膜外阻滞大鼠模型。实验组在切开置管后导管固定使用创新性关卡，且采用不透气无菌医用胶带封闭导管；对照组则采用单纯缝线环绕法固定，并保留导管接头固定于后颈部。术后每天硬膜外腔注射0.1%罗哌卡因30ul，共28d。对比观察两组术后发生感染率，硬膜外阻滞模型有效时间及硬膜外导管深度变化等情况。结果：术后感染发生率：两组间差异无统计学意义(P0.05)；模型有效时间：实验组长于对照组(26.2±1.7d 18.5±3.0d，P0.05)；硬膜外腔内导管深度：实验组与对照组比，其均值和离散程度的差异均具有显著性(1.81±0.07 1.44±0.55，P0.05)。结论：应用改良导管固定法构建大鼠硬膜外阻滞模型，有助于提高其稳定性和成功率。( 【关键词】 麻醉，硬膜外；模型，动物；大鼠 【中图分类号】 R614.4+2 Modified Catheter Fixed Method Used to Build Rat Epidural Anesthesia Models ZHOU Bo1, CHEN Guo-zhong1，3, LIU Xiao-long1, XU Xiu-yi2, LIU Ren3 Wang Li-ping3 Department of Anesthesiology，Clinical Medical College of Fuzhou General Hospital，Fujian Medical University，Fuzhou，350025，China；2.；Department of Anesthesiology，Maternal and Child Health Hospital，Xiamen，361003，China；3. Department of Anesthesiology，Fuzhou General Hospital of Nanjing Military Command，Fuzhou 350025，China) 【Abstract】 Objective To investigate the feasibility and effect of rat epidural anesthesia models by modifying fixation of the epidural catheter. Methods 24 male Wistar rats, used to build epidural anesthesia models, were randomly divided into experimental group (n = 12) and control group (n = 12). After inserted the catheter, the catheter were fixed by some blocks designed with sterile adhesive tape and the catheter connector were replaced by sterile medical tape in the experimental group, while catheter was fixed with wire wound and catheter connector was sutured on the back of the neck in the control group. 0.1% ropivacaine 30ul was injected into epidural space once a day and continued 28 days. Then incidence of postoperative infection, the time to failure of the epidural anesthesia models was recorded and the epidural catheter depth measured on the 28th day. Results No significant difference was noted in the incidence of