多壁碳纳米管浓度对原位合成纳米羟基磷灰石影响.doc

壳聚糖/多壁碳纳米管复合膜诱导羟基磷灰石仿生合成的研究 杨 俊1，陈治清2，胡常红1 （400015重庆，重庆医科大学附属口腔医院1；610041成都，四川大学华西口腔医学院2） [摘要] 目的 制备壳聚糖/多壁碳纳米管复合膜作为骨引导再生膜，诱导羟基磷灰石仿生合成，研究低浓度多壁碳纳米管对复合膜仿生矿化的影响。方法 壳聚糖/多壁碳纳米管复合膜在仿生溶液中仿生矿化，并用FTIR、SEM、XRD对复合膜表面的形态和结构进行表征，同时将复合膜分别与血清蛋白和大鼠骨髓基质干细胞培养。结果 壳聚糖/多壁碳纳米管复合膜表面矿化生成纳米羟基磷灰石，并且多壁碳纳米管的分散程度影响羟基磷灰石晶体的体积。当多壁碳纳米管的浓度为1％（质量百分比）时，羟基磷灰石晶体的体积最小，约9.6nm。多壁碳纳米管能够促进复合膜吸附蛋白（P0.05），并且在实验早期（2h），1%浓度吸附量最高。同时，碳管的添加能够促进大鼠骨髓基质干细胞增殖，1%浓度最为显著（P0.05）。结论 壳聚糖/多壁碳纳米管复合膜具有促进骨形成的潜力。 [关键词] 壳聚糖；多壁碳纳米管；原位合成；纳米羟基磷灰石 [中图法分类号] [文献标志码] A Research on the chitosan/multiwall carbon nanotube composites Yang Jun1,Chen zhiqing2, Hu Changhong1 (1Affiliated Hospital of Stomatology ,Chongqing Medical University,Chongqing, 400015;2West China College of Stomatology, Sichuan University, Chengdu, Sichuan Province, 610041, China) ［Abstract］ Objective To determine the osteogenic potential of the chitosan/multiwall carbon nanotube composites . Methods Calcium phosphate coating on the surface of chitosan/multiwall carbon nanotube (CS/MWNT) composites was induced through simulated body fluid.Fourier-transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction were used for characterization.The membranes were cultured with bovine serun protein solution and rat bone marrow stromal stem cells. Results Nanoscopic hydroxyapatite formed on the CS/MWNT composites at low MWNT concentrations, and the dispersion of the multiwall carbon nanotube affected the crystallite size of the nanoscopic hydroxyapatite, with the smallest size of 9.6 nm at 1.0% . The composites adsorped serum protein,and promoted cells proliferation, with best effect at 1.0% (P0.05).Conclusion The chitosan/multiwall carbon nanotube composites have the osteogenic potential. [Key words] chitosan; multiwall carbon nanotube;in-situ synthesis; nanoscopic hydroxyapatite Corresponding author: Hu Changhong, E-mail:eyjjjyy@ 牙槽骨的再生一直是全世界牙医面临的难题。引导骨组织再生(GTR)是解决这一难题的办法之一。理想的GTR膜不但有良好的机械性能还要能够引导骨组织的再生[1-2]。碳纳米管(CNTs)自从1991年问世以来