幼年及成年大鼠肠缺血-再灌注肠组织形态学对照-中国试验动物学报.DOCVIP

幼年及成年大鼠肠缺血-再灌注肠组织学，林春榕2，吴学东1，王敏1，李正金1，张杉杉1，黄勇1，李娜1 （大理学院1.附属医院暨临床医学研究中心, 2.基础医学院，云南大理，671000） [摘要] 目的 在建立小肠缺血再灌注intestinal ischemia-reperfusion?injury, II/R)模型的基础上，II/R幼年及成年大鼠肠形态。方法 4周龄10周龄SD大鼠，分幼鼠和成组，II/R模型，分别于、缺血1h、再灌注1h再灌注2h距回盲部5cm处肠管行进行Chiu’s氏6级评分，统计学分析。结果再灌注2肠绒毛上皮细胞脱落，溃疡面多，Chiu氏评分结果随缺血和再灌注时间的延长而评分增加，再灌注1h时幼鼠组的分值高于成鼠组（P0.05），其余各时间点两组评分值间的差异无统计学意义（P0.05）。结论 肠组织病理II/R的时间延长而加重，其中又以幼年大鼠肠损伤 缺血-再灌注；肠组织；大鼠Comparative morphological study about intestinal ischemia-reperfusion between young and adult rats HOU Jia-wei1, LIN Chun-rong2, WU Xue-dong1, WANG Min1, LI Zheng-jin1, ZHANG Shan-shan1, HUANG Yong1, LI Na1. Dali University (1. Affiliated Hospital ＆Corresponding author: WU Xue-dong, E-mail: xuedong3288@ [Abstract] Objective To explore the difference of intestinal histological changes induces by ischemia-reperfusion (I/R) between young and adult rats. Methods 40 SD rats were divided into young and adult groups each 20 with 4 and 10 weeks old respectively. Based on the I/R model of superior mesenteric artery occluded, the intestinal specimens were harvested before and after ischemia 1h, reperfusion 1h and 2h, Chiu’s scores were calculated according to their morphological changes were performed and the data analyzed statistically. Results There was pathological changes performance for chorionic villi lodging and hyperemia under villi space of intestine after ischemia. Villus necrosis and small ulcer formation, intrinsic layer separation can be found in intestine after reperfusion, there was the aggressive trend with the reperfusion time prolong especially in young group. But for the Chiu’s score, its higher in young group at reperfusion 1h than that in adult group only (P0.05), no significant difference the scores compared between two groups in another three observe time points ( P0.05 ). Conclusions Intestinal tissue damage induced by I/R is severer in young rat than adult and increased with reperfusion prolong. [Key words] Ischemia/reperfusion injury; Intestine; Morp