鲑鱼降钙素预防股骨头坏死的实验性研究-外科学专业论文.docxVIP

研究目的鲑鱼降钙素预防股骨头坏死的实验性研究摘要根据激素性股骨头坏死的骨质疏松理论，实验性研究治疗骨质疏松药物鲑鱼降 钙素对于早期激素性股骨头坏死的预防作用，并探讨其机制研究方法将 18 只成年健康 SD 大鼠随机分为 3 组，A 组：马血清+地塞米松，B 组：马 血清+地塞米松+鲑鱼降钙素+乐力钙，C 组：空白对照组 。A 组经尾静脉注射马血 清 20ml/kg·次，共两次，间隔 3 周，第二次注射马血清后两周，连续 3 天腹腔内注 射地塞米松注射液 20mg/kg·天；B 组除与 A 组相同部分外，另在注射马血清第 1 天开始肌肉注射鲑鱼降钙素 10u/kg·d，并乐力灌胃 250mg/kg·d；空白对照组于相 应时间相同部位注射和灌胃同等量生理盐水。研究结果A 组股骨头软骨下骨细胞有较为明显的骨细胞坏死，骨髓腔内大量散在脂肪细 胞，髓腔造血细胞活跃程度也有明显的减少，B 组损伤较轻，A，B 组均有骨坏死后 的修复，但 B 组更为明显。研究结论大剂量使用激素可以造成激素性股骨头坏死，早期联合应用鲑鱼降钙素+乐力 钙，对于早期的激素性股骨头坏死有一定的预防和促进其修复作用。 关键词：股骨头坏死；激素；鲑鱼降钙素The experimental research on Salmon calcitonin prevention of glucocorticoid-induced femoral head avascular necrosisAbstractObjectiveAccording to the theory of glucocorticoid-induced avascular necrosis of the osteoporosis, experimental research and treatment of osteoporosis drug salmon calcitonin for glucocorticoid-induced avascular necrosis of the early prevention, and to explore its mechanism.Methods18 healthy adult SD rats were randomly divided into three groups, Group A: horse serum + dexamethasone, group B: horse serum salmon calcitonin + le + dexamethasone + calcium, C groups: blank control group. Via tail vein injection group A horse serum 20 ml/kg·times, twice in total, and interval of 3 weeks, two weeks after the second round of horse serum injection, intraperitoneal injection of dexamethasone injection for three days 20 mg/kg·d; In addition to the part same as group A, group B in another horse serum 1 day intramuscular injection salmon calcitonin 10 u/kg·d, and Compound Calcium Amino Acid Chelate Capsules lavage 250 mg/kg·d; Blank control group in the corresponding time same parts injection and gastric same amount of saline solution.ResultsGroup A,under cartilage bone of femoral head cells have relatively obvious necrosis of bone cells, A large number offat cells scattered in the bone marrow cavity, medullary cavity hematopoietic cell activity has decreased significantly, the injury of group B is lighter, group A and B hadrepaired after osteonecrisis ,