表达HPV16 L1、L2和E7蛋白的非复制重组痘苗病毒的构建.pdfVIP

肿瘤防治研究2008年第35卷第4期 ·基础研究· 6 表达HPVlL1、L2和E7蛋白的非复制重组 痘苗病毒的构建 范江涛1，陈心秋2，黄 薇2，田厚文3 Constructionof RecombinantVacciniaVirus HPVl6L1．L2E7 Non-replicating Expressing Proteins FAN Wei2，TIANHou-wen3 Jiang-ta01，CHENXin-qiu2，HUANG First Medical of AffiliatedHospital，GuangxiUniversity，Nanning 1．DepartmentGynecology，The Tumor，The 530021，China；2．DepartmentofGynecologicalAf豇liatedOncologyHospitalofGuangxi Medical CenterDiseaseControlandPrevention University；3．InstituteofVirology，Chinesefor Toconstructa vacciniavirus HPVl6 Abstract：Objective non-replicatingexpressingL1，L2E7proteins vaccineforcervicalcancer．Methodsvaeeiniavirus astrainof asacandidate vector。we Using generated recombinantvacciniavirusvaccine HPVl6L1，L2E7 non-replicating expressing proteinsbyhomologous andidentifiedPCRandWestern Wedemonstratedthatthe recombination by blot．Results L1，L2E7 ofHPVl6were intovaccinia andcould L1，L2E7 when gene integrated genosome express proteinstably infectedtheCEF PCRandWesternblot can using assay．ConclusionNTVJLl／L2E7expressL1，L2E7 ofHPVl6andcanbetakenasflcandidatevaccineforHPVl6associated protein diseases． Keywords：HurnanPapillomavirus；Vacciniavirus；Vaccine 摘要：目的 构建防治宫颈癌的表述人乳头瘤病毒16型(HPVl6)L1，L2E7蛋白的非复制重组痘苗 L1，L2E7蛋白的重组 病毒疫苗株。方法以痘苗病毒为载体，利用同源重组技术筛选共表迭HPVl6 痘苗病毒，用PCR和Westernblot技术进行鉴定。结果该病毒在鸡胚成纤维细胞(CEF)中连续传代 15代，经PCR检测证实病毒基因组中有HPVl6L1，L2E7目的基因插入，Westemblot检测证实病毒可 L1和 以稳定