毕业论文-川芎药用成分的提取及含量测定.doc

川芎药用成分的提取及含量测定 摘要：川芎芎穷、西芎、、、小叶川芎等。科属为伞形科藁本属，多年生草本临床上用于治疗心脑血管系统、血液系统疾病本实验以含量较高的活性成分阿魏酸为评判指标，先对提取溶剂和提取方法进行筛选，然后以溶剂浓度、溶剂加量和提取时间为考察因素进行用正交实验对川芎的提取工艺条件进行优化。结果表明，川芎中提取阿魏酸，用乙醇作为溶剂要优于甲醇、丙酮，以热回流法提取优于超声提取法、索氏提取法。正交设计实验表明最佳提取工艺条件为10g川芎生药粉用150ml的80%乙醇提取3h。 本实验对筛选出的最佳工艺条件进行了验证，表明了筛选出的最佳工艺条件具有一定的可行性，具有较高的稳定性，而且所提取的有效成分含量比其他方法高。 关键词：川芎阿魏酸正交设计最佳工艺含量 Extraction and Assaying of Medicinal ingredients in Ligusticum Chuanxiong Hort Abstract: Chuanxiong was also named Xiongqiong, Xixiong, Jingxiong,Duxiong, Foliolum Chuanxiong. The Branch of Chuanxiong is Umbelliferae Ligusticum and Chuanxiong is a perennial herb. The medicinal part of Chuanxiong is rhizome, Modern clinical practice for the treatment of cardio-cerebral vascular system, blood system diseases and so on. In this experiment with higher levels of the active ingredient of ferulic acid for evaluation indicators, First screening the extraction solvent and extraction methods, And then with the dissolvent concentration, dissolvent volume and extraction time for the study of factors to optimized extraction conditions of Chuanxiong by orthogonal test. The results showed that The ferulic acid extracted from Chuanxiong , ethanol is superior to methanol,acetone as a solvent, heat reflux extraction method is superior to ultrasonic extraction, Soxhlet extraction method.The optinmm extraction processes of extracting ferulic acid from Chuanxiong was adding 150ml of 80％ alcohol，refluxing and extracting 3 hour for 10g. This experiment verify to the optimum conditions,showed that optimum conditions possess the feasibility , certain high stability, and the extraction of active ingredients than any other method. Key words：chuanxiong，ferulaic acid，orthogonal design，optimization artwork，contents 目 录 第1章 绪 论 1 1.1川芎的概述 1 1.1.1 川芎的植物形态 1 1.1.2 川芎的种植 2 1.1.3 川芎的采收与加工 2 1.1.4 川芎的鉴别 3 1.2川芎的本草考证 4 1.3川芎的化学成分及药理作用研究现状 4 1.3.1 川芎化学成分的研究 4 1.3.2 川芎药理作用的研究 5 1.3.3 川芎的发展前景 8 1.4提取方法的介绍 9 1.4.1 超声波提取技术 9 1.4.2 加热回流提取法 9 1.4.3 索氏提取法