蛋白质相互作用的生物信息学幻灯片.ppt

Overbeek R PNAS 96, 2896 (1999) Phylogenetic profile PNAS (1999) 96, 4285-4288 A Combined Experimental and Computational Strategy 1) Screen random peptide libraries by phage display to define the consensus sequences for preferred ligands that bind to each peptide recognition module. 2) On the basis of these consensus sequences, computationally derive a protein-protein interaction network that links each peptide recognition module to proteins containing a preferred peptide ligand. Science 2002 295, 321 3) Experimentally derive a protein-protein interaction network by testing each peptide recognition module for association to each protein of the inferred proteome in the yeast two-hybrid system. 4) Determine the intersection of the predicted and experimental networks and test in vivo the biological relevance of key interactions within this set. A Combined Experimental and Computational Strategy Science 2002 295, 321 高友鹤 gaoyouhe@pumc.edu.cn * mRNA levels are systematically measured under a variety of different cellular conditions, and genes are grouped if they show a similar transcriptional response to these conditions. * Two nonessential genes that cause lethality when mutated at the same time form a synthetic lethal interaction. Such genes are often functionally associated and their encoded proteins may also interact physically. This type of genetic interaction is currently being studied in an all-versus-all approach in yeast9 . * Figure 1. BIND Interaction Viewer Java applet showing how molecules can be connected in the database from molecular complex to small molecule. Yellow, protein; purple, small molecule; white, molecular complex; red, a square is fixed in place and will not be moved by the graph layout algorithm. This session was seeded by the interaction between human LAT and Grb2 proteins involved in cell signaling in the T-cell. * Figure?1.?Network cross-comparison.Pairs of proteins have been binned according to their shortest path in networks gener