桑寄生中总黄酮的含量测定及抗氧化活性研究.pdfVIP

2012 年3 月 食品研究与开发 第33 卷第3 期 Food Research And Development 检测分析 120 桑寄生中总黄酮的含量测定及抗氧化活性研究 杨再波，杨胜峦，龙成梅，毛海立，孙成斌 （贵州省黔南民族师范学院化学与化工系，贵州都匀558000） 摘 要：建立桂花树桑寄生中总黄酮的紫外分光光度测定方法；用清除DPPH 自由基的能力来评价桑寄生中总黄酮的 抗氧化活性。总黄酮浓度在0.004 mg/mL~0.02 mg/mL 范围内，吸光度与浓度呈具有良好的线性关系(r=0.999 5)。桂花树桑 寄生中总黄酮含量为14.18 mg/g，平均加标回收率为101.2 ％，同一批样品5 次测定值的RSD 为2.07 ％~2.25 ％之间；抑 制DPPH 自由基的能力IC50 是32.31 μg/mL 。该方法简便、快捷、准确、重现性好，可作为检测桂花树桑寄生中总黄酮含 量的一种好方法；结果表明，桂花树桑寄生总黄酮具有一定的抗氧化活性。 关键词：桂花；桑寄生；总黄酮；紫外分光光度计；DPPH Determination of the Total Flavonoids and Antioxidant Activity of Taxillus chinensis YANG Zai-bo, YANG Sheng-luan, LONG Cheng-mei, MAO Hai-li, SUN Cheng-bin （Department of Chemistry and Chemical Engineering, Qiannan Normal University for Nationalities, Duyun 558000, Guizhou,China） Abstract ：The spectrophotometry was used to determine the content of flavonoid in Taxillus chinensis (DC.) Danser；the antioxidant activity of flavonoids in Taxillus chinensis was evaluated using DPPH free radical. Absorbance has good linear relation with flavonoid strength in the range of 0.004 mg/mL-0.02 mg/mL(r=0.999 5). T he total flavones content in samples was 14.18 mg/g calculated by rutin ，the recovery was 101.2 ％, The assayed value of RSD after 5-time determination from the same samples was between 2.07 ％ and 2.25 ％. DPPH radical inhibition capacity of IC50 was 32.31 μg/mL. The method was simple, rapid, accurate and has good reproducibility,which can be used to determine the content of total flavones in Taxillus chinensis . The results showed that the total flavonoids in Taxillus chinensis has some antioxidant activity. Key words ：osman