可视化猪肺炎支原体lamp检测方法的建立-上海动物研究中心.pdfVIP

中国动物传染病学报 2017,25(4): 29-33 Chinese Journal of Animal Infectious Diseases ·研究论文· 可视化猪肺炎支原体 LAMP 检测方法的建立 熊 炜 1 ，王 艳 1 ，魏晓锋2 ，李春阳 1 ，张 强 1 ，黄忠荣 1 ，胡建华2 ，李 健 1 （1. 上海出入境检验检疫局，上海 200135 ；2. 上海实验动物研究中心，上海 201203 ） 摘 要：由猪肺炎支原体（Mycoplasma hyopneumoniae ）引起的慢性肺炎是最难净化的疫病之一，它流行广、感染率高，对养猪 业危害巨大。为了加强口岸现场查验动物源性产品中猪肺炎支原体的力度，本研究建立了猪肺炎支原体环介导等温核酸扩增技 术（Loop-mediated isothermal amplification ，LAMP ）检测方法。本研究通过在LAMP反应产物中加入显色液，提高了检测结果 肉眼判定的准确性，实现了结果判定可视化的目标，有助于出入境口岸货物查验现场及猪场中猪肺炎支原体的检测。 关键词：猪肺炎支原体；LAMP ；可视化检测 中图分类号：S852.62 文献标志码：A 文章编号：1674-6422 （2017 ）04-0029-05 ESTABLISHMENT OF VISIBLE LAMP METHODS FOR MYCOPLASMA HYOPNEUMONIAE 1 1 2 1 1 1 XIONG Wei , WANG Yan , WEI Xiao-feng , Li Chun-yang , ZHANG Qiang , HUANG Zhong-rong , 2 1 HU Jian-hua , LI Jian (1.Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China; 2.Shanghai Laboratory Animal Research center, Shanghai 201203, China) Abstract: Mycoplasma hyopneumoniae causes chronic pneumonia among pigs, which is one of hard erased diseases with characters of wide spread and high infection. In order to survey and detect Mycoplasma hyopneumoniae carried by animal original products at entry- exit port, a Loop-mediated isothermal amplification (LAMP) method was established and proved to be specific with high sensitivity and stability. By adding SybrGreen into LAMP product liquid, detection results could be read easily by eyes, which is more convenient to be used by ports and basic laboratories. Key words: Mycoplasma hyopneumoniae ；loop-mediated isothermal amplification (LAMP) ；visible detection 猪支