β-葡萄糖苷酶基因工程菌发酵工艺优化.pdfVIP

※生物工程 食品科学 2010, Vol. 31, No. 11 173 β- 葡萄糖苷酶基因工程菌发酵工艺优化 1 1 1 1 2 刘长江 ，梁 爽 ，郑 艳 ，宗绪岩 ，李长彪 ( 1.沈阳农业大学食品学院，辽宁沈阳 110 16 1；2 .抚顺市农村经济委员会，辽宁抚顺 113000) 摘 要：以菌体生物量和酶比活力为参考指标，通过单因素和响应面分析法优化β- 葡萄糖苷酶基因工程菌发酵条 件，得到最优的发酵条件：温度3 7 .9 ℃、p H 6 .6 、接种量10 % 、转速2 0 0r/ m in 、诱导时间10h ，在此条件下发 酵可以使β- 葡萄糖苷酶酶活力达到14 7.8U/L 。 关键词：β- 葡萄糖苷酶；基因工程；发酵工艺 β-Glucosidase Production under Optimized Fermentation Conditions of Genetically Engineered Bacterial Strain 1 1 1 1 2 LIU Chan g-j iang ，LIANG Shuang ，ZHENG Yan ，ZONG Xu-yan ，LI Chan g-biao (1. College of Food Science, Shenyang Agricultural University, Shenyang 110 16 1, China ； 2. Committee of Rural Economy of Fushun , Fushun 113000, China) Abstract ：The fermentation conditions of genetically engineered bacterial strain for β-glucosidase production were optimized using one-factor-at-a-time method and respon se surface analysis based on central composite design . The optimal procedure for β-glucosidase production was based on 8 h fermentation under the conditions of pH 6.6, 37.9 ℃, 10% inoculation amount, 200 r/min rotation speed followed by 10 h of induction with the addition of 0.5 mmol/L IPTG, which made the enzyme activity and of β-glucosidase reach up to 147.8 U/mL, respectively. Key words ：β-glucosidase ；genetically engineered bacterial strain ；fermentation process 中图分类号：Q784 文献标识码：A 文章编号：1002-6630(20 10) 11-0 173-05 β- 葡萄糖苷酶(EC3,2,1,2 1) ，又称为β-D- 葡萄糖苷 麦、蜀黍、旱金莲、长春花等植物中进行了克隆和表