二氢黄酮桔皮素对MCF-7细胞凋亡的影响.pptVIP

Effect Of Flavonone Hesperidin On The Apoptosis Of MCF-7 Investigate the impact of flavonone hesperidin on MCF-7. Flavonone hesperidin’s role in induction of apoptosis. 2、Introduction Breast cancer is the 3rd cause of death in worldwide. Need for development of new anticancer drugs to effectively treat. However , current treatments( radiation, anti-hormonal therapy , etc) have side effects. Hence , an effective chemotherapeutic agents is important to improve the survival rate and is an need for treatment. Chemotherapeutic Agents Low side effect. Low toxicity. Neutralization of carcinogenic agents. Has its effects on cells line (MCF-7). 3、Methods 96-well microplates, 1*10 cells/well . Serial concentrations : 20, 40, 60, 80, 100uM, 24H. MTT, 4H. DMSO , overnight. Measure the absorbance at 540nm. The reliable to detect proliferation of cells. Widely used to quantify cell viability and proliferation. The clearly proved that as a bioflavonoid hesperidin strongly inhibits the MCF-7 cells. Ⅱ、LDH The concentrations are added to cells. Sample are added to KITS. Measured at 340nm for 45 seconds. LDH levels have been increased significantly in hesperidin treated MCF-7 cells when compared to control cells. LDH leakage on MCF-7 may due to the cytotoxic nature of the drug. Ⅲ、GSH 5% TCA is added to MCF-7cells. The DTNB is added to supernatant. The absorbance is read at 412nm. GSH : Important cellular antioxidant and a major role in protecting cells against oxidative stress. Reduce the intracellular GSH level leads to the formation of reactive oxygen species in cells treated with antitumor drugs, which in turn leads to DNA damage and cell death. It suggests that the decrease in GSH level may initiate redox imbalance in MCF-7 cells and subsequently induce apoptosis. Ⅳ、Light Microscopic Study Observe the morphological changes after treatment with F-hesperidin for 24H. Showed the typical morphological features of apoptosis in MCF-7 cells that the destruction of monolayer, c